MagCellect CD24- CD44+ Breast Cancer Stem Cell Isolation Kit

Methods that use both positive and negative selection to isolate cancer stem cells can yield cells with a higher purity.

R&D Systems MagCellect CD24^- CD44⁺ Breast Cancer Stem Cell Isolation Kit (Catalog # MAGH111) enriches for a small population of human breast cancer cells, which can form new tumors in mice. This kit uses a two-step procedure that combines negative and positive selection techniques. Briefly, CD24^low/– cells are initially enriched by negative selection whereby unwanted CD24⁺ cells are tagged and magnetically removed. CD44⁺ cells are subsequently isolated from the CD24^low/– population by positive selection using a biotinylated human CD44 antibody, streptavidin-conjugated magnetic beads, and a MagCellect magnet (Catalog # MAG997, or equivalent). The efficiency of enrichment can be assessed by staining the recovered cells with fluorochrome-conjugated anti-human CD24 and CD44 detection antibodies which are also provided in the kit.

The MagCellect CD24^- CD44⁺ Breast Cancer Stem Cell Isolation Kit:

• Isolates CD24^low/- CD44⁺ cells which have been shown to form tumors in mice.
• Uses positive and negative selection to increase the purity of isolated cells.
• Includes fluorochrome-conjugated CD24 and CD44 antibodies for analysis of recovered cells.