GMP Cloudz Human Treg Cell Expansion Kit GMP

Fold Expansion of Treg Cells using the GMP Cloudz Human Treg Cell Expansion Kit Primary human CD4⁺ cells were activated with GMP-Grade Cloudz Treg CD3/CD28 and cultured for 9 days in ExCellerate T Cell Expansion Media, Xeno-Free (R&D Systems, Catalog # CCM030) and 400 IU/mL of IL-2 (R&D systems, Catalog # 202-GMP). Expansion of FoxP3⁺ Treg cells was evaluated using flow cytometry on Day 5 and Day 9 and fold expansion of was calculated relative to Day 0. Error bars reflect standard deviation across 3 donors.

Fold Expansion of CD4⁺/CD25⁺/CD127^- Treg Cells using the GMP Cloudz Human Treg Cell Expansion Kit Primary human CD4⁺ cells were activated with GMP-Grade Cloudz Treg CD3/CD28 and cultured for 9 days in ExCellerate T Cell Expansion Media, Xeno-Free (R&D Systems, Catalog # CCM030) and 400 IU/mL of IL-2 (R&D systems, Catalog # 202-GMP). Expansion of CD4⁺/CD25⁺/CD127^- Treg cells was evaluated using flow cytometry on Day 5, 7 and 9 and fold expansion of was calculated relative to Day 0. Error bars reflect standard deviation across 3 donors.

Increased FoxP3⁺ CD4 Cells following Treg Expansion. Flow cytometry data showing expression of the Treg cell marker, FoxP3, in CD4⁺ T cells on Day 0 (A, C) and Day 9 (B, D) following expansion using the GMP Cloudz Human Treg Expansion Kit. Nine days after expansion, the cells were fixed, permeabilized, and stained with CD4-FITC, CD25-APC, and FoxP3-PE antibodies. Cells in panels A and B were stained with FoxP3 and CD4. Cells in C and D were stained with FoxP3 and CD25.

Viability greater than 90% after 9-day expansion Viability of Human Treg after expansion with GMP Cloudz Human Treg Cell Expansion Kit. Primary human CD4⁺ cells were activated with GMP-Grade Cloudz Treg CD3/28 and cultured for 9 days in ExCellerate T Cell Expansion Media, Xeno-Free (R&D Systems, Catalog # CCM030) and 400 IU/mL of IL-2 (R&D systems, Catalog # 202-GMP). Cell viability after expansion for 9 days was greater than 90%. Error bars reflect standard deviation across 3 donors.

Fold expansion of Treg Cells with GMP Cloudz Human Treg Cell Expansion Kit compared to other suppliers Fold expansion of CD25⁺/CD4⁺/FoxP3⁺ Treg Cells is greater with GMP Cloudz Human Treg Cell Expansion Kit compared to other GMP-Grade Treg Cell Expansion suppliers.