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Cultrex Rat Collagen I

Purified stromal extracellular matrix protein that promotes cell adhesion for cell and tissue cultures

Key Product Details

Features: Cultrex Rat Collagen I is a purified stromal extracellular matrix protein that promotes cell adhesion for cell and tissue culture. It may also be used to supplement customized coatings, hydrogels, bioinks, or medium formulations for cell culture.
Key Benefits:
  • Qualified for general cell culture applications
  • May be used as a hydrogel or thin coating for tissue culture vessels
  • Material provided at a standard concentration of ~5 mg/mL
Cultrex Rat Collagen I
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3440-100-01
3440-005-01

Assay Procedure

Citations for Cultrex Rat Collagen I (36)

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FAQs

  • What is the difference between the standard Cultrex Rat Collagen I and the Cultrex 3-D Rat Collagen I?

    Cultrex 3-D Rat Collagen I undergoes the same basic purification and efficacy tests as the standard collagen. However, it undergoes additional 3-D culture validation; it has been tested extensively for the ability to promote growth and differentiation of cell types, visualized by morphology in three dimensions in vitro.

  • What are the differences between Cultrex Rat Collagen I and Cultrex Rat Collagen I, Low Viscosity?

    Cultrex Rat Collagen I is provided at 5 mg/mL and is not pepsin treated, so it has intact telopeptides and is highly viscous. Cultrex Rat Collagen I, Low Viscosity is provided at 3 mg/mL, and it is easier to pipet. Both are provided in acetic acid and require pH neutralization prior to use. Please consult the provided product data sheets for pH neutralization instructions.

  • Is Cultrex Rat Collagen I atelocollagen or telocollagen?

    Cultrex Rat Collagen I is telocollagen.  

  • After coating the Cultrex Rat Collagen I (catalog # 3440-100-01), there is a white film on the plate. What is this white film? Is this normal?

    This white film is normal. The collagen coating can result in a slight white haziness to the plate, which could also be the result of excess salt if the plates are not well aspirated.

  • Can the Organoid Harvesting Solution, Catalog # 3700-100-01,  be used to dissociate cells grown on a substrate of Cultrex Rat Collagen 1?

    The Organoid Harvesting Solution works well with BME (Basement Membrane Extract).  For cells grown on Cultrex Rat Collagen 1, the recommended enzyme to use to dissociate cells is Collagenase. Collagenase treatment at 37oC for 30 minutes is recommended.

Product Documents

Certificate of Analysis

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