What is the difference between the standard Cultrex Rat Collagen I and the Cultrex 3-D Rat Collagen I?

Cultrex 3-D Rat Collagen I undergoes the same basic purification and efficacy tests as the standard collagen. However, it undergoes additional 3-D culture validation; it has been tested extensively for the ability to promote growth and differentiation of cell types, visualized by morphology in three dimensions in vitro.

What are the differences between Cultrex Rat Collagen I and Cultrex Rat Collagen I, Low Viscosity?

Cultrex Rat Collagen I is provided at 5 mg/mL and is not pepsin treated, so it has intact telopeptides and is highly viscous. Cultrex Rat Collagen I, Low Viscosity is provided at 3 mg/mL, and it is easier to pipet. Both are provided in acetic acid and require pH neutralization prior to use. Please consult the provided product data sheets for pH neutralization instructions.

Is Cultrex Rat Collagen I atelocollagen or telocollagen?

Cultrex Rat Collagen I is telocollagen.

Cultrex Rat Collagen I, Lower Viscosity

Name: Cultrex Rat Collagen I, Lower Viscosity
Brand: R&D Systems
SKU: 3443-100-01

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 3443-100-01

Citations (6)

Product Datasheet / COA / SDS

Purified stromal extracellular matrix protein that can promte cell adhesion for cell and tissue cultures

Key Product Details

Features:	Cultrex Rat Collagen I, Low Viscosity is a purified stromal extracellular matrix protein that is formulated as an easy to work with hydrogel for coating tissue culture vessels. It may also be used to supplement customized coatings, hydrogels, or medium formulations for cell culture.
Key Benefits:	Qualified for general cell culture applications Lower in viscosity for easier handling May be used as a hydrogel or thin coating for tissue culture vessels Material provided at a standard concentration of ~3 mg/mL

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Setting the standard in quality research reagents

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Citations (6) / Reviews / FAQs

Summary for Cultrex Rat Collagen I, Lower Viscosity

Cultrex Rat Collagen I, Lower Viscosity

Why Use Cultrex Rat Collagen I, Lower Viscosity?

Cultrex Rat Collagen I, Lower Viscosity is purified from rat tail tendons and is provided at a standard concentration that is lower in viscosity, making it easier to work with. Collagen I is the major structural component of extracellular matrices (ECM) found in connective tissue and internal organs, but is most prevalent in the dermis, tendons, and bone. It is a 300 kDa molecule composed of two alpha1(I) chains and one alpha2(I) chain that spontaneously forms a triple helix scaffold at a neutral pH and 37°C. This phenomenon can be exploited to promote cell attachment, growth, differentiation, migration, and tissue morphogenesis during development. To provide the most standardized Collagen I, a special process is employed to provide material at a standard concentration of approximately 3 mg/mL.

Product Specifications for Cultrex Rat Collagen I, Lower Viscosity

Species	Rat
Source	Rat tail tendons
Protein Concentration	3 mg/mL
Endotoxin level	≤ 20 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing	No bacterial or fungal growth detected following 14 days in culture
Cell Culture Testing	Supports cell attachment and spreading of HT1080 human fibrosarcoma cells.
Viral Testing	No mycoplasma contamination detected by PCR.

Preparation & Storage

Stability	Product is stable for a minimum of 2 months if stored at 2-8 °C
Shipping Conditions	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage	Store the unopened product at 2 - 8 °C. Do not freeze.

Citations for Cultrex Rat Collagen I, Lower Viscosity (6)

Showing 1 - 6 of 6 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Cultrex Rat Collagen I, Lower Viscosity include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

? Suprewicz et al. (2022), Recombinant human plasma gelsolin reverses increased permeability of the blood-brain barrier induced by the spike protein of the SARS-CoV-2 virus Journal of Neuroinflammation, 19(1):282.
PMID: 36434734
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

LA Marquez-Cu et al. (2021), Cryopreservation of human cerebral microvascular endothelial cells and astrocytes in suspension and monolayers PLoS ONE, 16(4):e0249814.
PMID: 33852594
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

E Colucci-Gu et al. (2020), Spatiotemporal analysis of mycolactone distribution in vivo reveals partial diffusion in the central nervous system PloS Neglected Tropical Diseases, 14(12):e0008878.
PMID: 33264290
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Pinto et al. (2019), Boosting Drug Discovery for Parkinson's: Enhancement of the Delivery of a Monoamine Oxidase-B Inhibitor by Brain-Targeted PEGylated Polycaprolactone-Based Nanoparticles Pharmaceutics, 11(7).
PMID: 31336891
Species: Human
Sample Types: Whole Cells

A da Costa et al. (2018), Innovative in cellulo method as an alternative to in vivo neurovirulence test for the characterization and quality control of human live Yellow Fever virus vaccines: A pilot study Biologicals, 0(0).
PMID: 29580693
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

K Tam et al. (2018), Assessing the Impact of Targeting CEACAM1 in Head and Neck Squamous Cell Carcinoma Otolaryngol Head Neck Surg, 0(0):1945998187566.
PMID: 29436278

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Showing 1 - 6 of 6 citations Showing All

FAQs for Cultrex Rat Collagen I, Lower Viscosity

What is the difference between the standard Cultrex Rat Collagen I and the Cultrex 3-D Rat Collagen I?

Cultrex 3-D Rat Collagen I undergoes the same basic purification and efficacy tests as the standard collagen. However, it undergoes additional 3-D culture validation; it has been tested extensively for the ability to promote growth and differentiation of cell types, visualized by morphology in three dimensions in vitro.
What are the differences between Cultrex Rat Collagen I and Cultrex Rat Collagen I, Low Viscosity?

Cultrex Rat Collagen I is provided at 5 mg/mL and is not pepsin treated, so it has intact telopeptides and is highly viscous. Cultrex Rat Collagen I, Low Viscosity is provided at 3 mg/mL, and it is easier to pipet. Both are provided in acetic acid and require pH neutralization prior to use. Please consult the provided product data sheets for pH neutralization instructions.
Is Cultrex Rat Collagen I atelocollagen or telocollagen?

Cultrex Rat Collagen I is telocollagen.

Cultrex Rat Collagen I, Lower Viscosity

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Summary for Cultrex Rat Collagen I, Lower Viscosity

Why Use Cultrex Rat Collagen I, Lower Viscosity?

Product Specifications for Cultrex Rat Collagen I, Lower Viscosity

Preparation & Storage

Citations for Cultrex Rat Collagen I, Lower Viscosity (6)

FAQs for Cultrex Rat Collagen I, Lower Viscosity

Product Documents for Cultrex Rat Collagen I, Lower Viscosity

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