Cultrex 3-D Spheroid Basement Membrane Extract Cell Invasion Assay, 96-well

Embedding Spheroids in Cultrex BME for Cell Invasion and Migration Assays. MDA-MB-231 (3,000 cells/well) formed spheroids in low adhesion plates for 72 hours using reagents in the Cultrex 3-D Spheroid Basement Membrane Extract Cell Invasion Assay. Briefly MDA-MB-231 cells were cultured in the presence of 1X Spheroid Formation Matrix. Then spheroids were embedded in A) no Invasion Matrix, or B) Spheroid Invasion Matrix. After hydrogel polymerization, DMEM and 10% FBS was added to each well to promote a chemotactic response over a 96 hour period. Note the addition of the Spheroid Invasion Matrix enhanced cell invasion over spheroids cultured without embedding in the invasion matrix.