Cultrex 3-D Culture Matrix Laminin I Best Seller
R&D Systems, part of Bio-Techne | Catalog # 3446-005-01
Key Product Details
Features
Cultrex 3-D Culture Matrix Laminin I is an extracellular matrix hydrogel that directs cells to grow in three dimensions and assemble into organotypic structures in vitro.
Species
Mouse
Product Summary for Cultrex 3-D Culture Matrix Laminin I
Why Use Cultrex 3-D Culture Matrix Laminin I?
Cultrex 3-D Culture Matrix Laminin I is purified from Engelbreth-Holm-Swarm (EHS) tumor and is provided at a high concentration that is capable of polymerizing to form a hydrogel at 37°C. Laminin I is a major component of the basement membrane which is a continuous sheet of specialized extracellular matrix that forms an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that plays an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. Cultrex 3-D Culture Matrix Laminin I is a purified basement membrane protein that has been developed, produced and qualified specifically for use in 3-D culture studies. Cultrex 3-D Culture Matrix Laminin I may also be used as to supplement customized hydrogel or medium formulations for cell culture.
Negative for the presence of bacteria and fungi.
Negative for the presence of bacteria and fungi.
Product Specifications
Source
Murine Engelbreth-Holm-Swarm (EHS) tumor
Protein Concentration
6 mg/mL
Endotoxin Level
≤ 20 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing
No bacterial or fungal growth detected following 14 days in culture
Cell Culture Testing
3-D Culture - Laminin I Supports differentiation of a human epithelial cell line derived from mammary gland (MCF-10A) into acinar structures.
Cell Attachment - Tested for the ability to Supports cell attachment and spreading of MG63 human osteosarcoma cells.
Cell Attachment - Tested for the ability to Supports cell attachment and spreading of MG63 human osteosarcoma cells.
Formulation, Preparation, and Storage
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store at ≤ -20 °C in a manual defrost freezer. For optimal stability, store at ≤ -70 °C. Avoid freeze-thaw cycles
Stability
Product is stable for a minimum of 3 months from date of shipment. See lot specific Certificate of Analysis for expiration date.
Background: Laminin-1
Laminins are heterotrimeric, noncollagenous glycoproteins composed of combinations of five known alpha, four beta and three gamma chains in mammals. One alpha, beta and gamma subunit self-assembles to form at least 15 coiled-coil heterotrimers. Through specific interactions with integrins, dystroglycan, and other receptors, laminins contribute to cell differentation, cell shape and migration, maintenance of tissue phenotypes and survival. Some subunits, such as Laminin gamma1 (Laminin B2), are present in several laminins; Laminin-1 (EHS laminin), Laminin-2 (merosin), Laminin-3 (S-laminin), Laminin-4 (S-merosin), Laminin-6 (K-laminin) and Laminin-7 (KS-laminin). Others, such as the beta3 and gamma2 subunits, occur in only one known combination (e.g. with alpha3 in Laminin-5 / Epiligrin).
Alternate Names
Laminin-111, Laminin1
Additional Laminin-1 Products
Product Documents for Cultrex 3-D Culture Matrix Laminin I
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