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CellXVivo Human NK Cell Expansion Kit

For the expansion of human CD3-CD56+ NK Cells from peripheral blood mononuclear cells (PBMCs).

Key Product Details

Human NK Cell Expansion Using the CellXVivo™ Kit is Equivalent to Standard Feeder Cell Expansion. 
(6)
Kit-expanded NK Cells are Cytotoxic to Tumor Cells.
Flow Cytometry Analysis of Expanded Human NK Cells. 
Human NK Cells Expanded using the CellXVivo™ Kit Express NK Cell-specific Markers. 
Human NK Cells Expanded using the CellXVivo™ Kit Express High Levels of CD16.
CellXVivo Human NK Cell Differentiation Kit
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CDK015

Assay Procedure

Refer to the product datasheet for complete product details.

Briefly, CD3-CD56+ Natural Killer (NK) cells are expanded ex vivo from peripheral blood mononuclear cells with the CellXVivo Human NK Cell Expansion Kit using the following procedure:

  • Isolate PBMC from human blood
  • Culture PBMCs in Human NK Cell Expansion Media
  • Verify NK cell expansion after 14 days in culture
 

 

Reagents Provided

Reagents Supplied in the CellXVivo Human NK Cell Expansion Kit (Catalog # CDK015):

  • Human Killer Cell Base Media 2
  • NK Cell Expander 1
  • NK Cell Expander 2
  • NK Cell Expander 3
  • NK Cell Expander 4
  • NK Cell Expander 5
  • Reconstitution Buffer 1
  • Reconstitution Buffer 2
  • 20X Wash Buffer

 

Other Supplies Required

Reagents

  • Ficoll-Hypaque

Equipment

  • 75-cm2 cell culture flasks
  • Microscope
  • Hemocytometer
  • 37 °C and 5% CO2 humidified cell culture incubator
  • Centrifuge
  • Pipettes and pipette tips
  • Sterile deionized water

 

Procedure Overview

Protocol for NK Cell Expansion using the CellXVivo Human NK Cell Expansion Kit.

Isolate PBMCs from human blood.

Isolate PBMCs from human blood.

Perform a cell count.

Perform a cell count.

Suspend 1 x 106 PBMCs/mL in Human NK Cell Expansion Media.

 

Culture the cells for a total of 14 days.

 

Refresh media and split cells on day 4, 7, and 10.

Culture the cells for a total of 21 days.

Verify expanded NK cells by analyzing NK cell marker expression via flow cytometry (optional).

 

NK cells are ready to be used for downstream applications.

Verify expanded NK cells by analyzing NK cell marker expression via flow cytometry (optional)

Citations

Currently there are no citations for CellXVivo Human NK Cell Expansion Kit.

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    Name: Anonymous
    Verified Customer | Posted 12/11/2019
    CellXVivo Human NK Cell Expansion Kit Serum CDK015

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FAQs

  • Is there an advantage of using the Cloudz Human NK Cell Expansion Kit, Catalog # CLD004 over using the CelXVivo Human NK Cell Expansion Kit, Catalog # CDK015?

    In side by side comparison of Catalog # CLD004 and Catalog # CDK015, the fold-exapnsion of NK cells  was found to be  better with Catalog # CLD004.

Product Documents

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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