ONO AE3 208

Product Description

ONO AE3 208 is a high affinity and selective EP₄ receptor antagonist (K_i values are 1.3, 30, 790 and 2400 nM for EP₄, EP₃, FP and TP receptors respectively). Displays no affinity for EP₁, EP₂, DP or IP receptors (K_i >10 μM). Inhibits PGE₂-induced IL-8 production in colonic epithelial caco-2 cells and attenuates PGE₂ inhibition of natural killer T cell activation. Suppresses recovery from experimentally-induced colitis and stimulates CD4⁺ T cell proliferation in C57BL/6 mice. Also reduces metastasis of mammary tumor cells in a murine model of breast cancer. Orally active.

Solubility

Preparing Stock Solutions for ONO AE3 208

The following data is based on the product molecular weight 404.43

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.47 mL	12.36 mL	24.73 mL
5 mM	0.49 mL	2.47 mL	4.95 mL
10 mM	0.25 mL	1.24 mL	2.47 mL
50 mM	0.05 mL	0.25 mL	0.49 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Deng Exosome-like nanoparticles from intestinal mucosal cells carry prostaglandin E2 and suppress activation of liver NKT cells. J.Immunol. 2013 PMID: 23467936
• Dey Prostaglandin E₂ couples through EP₄ prostanoid receptors to induce IL-8 production in human colonic epithelial cell lines. Br.J.Pharmacol 2009 PMID: 19175605
• Kabashima The prostaglandin receptor EP₄ suppresses colitis, mucosal damage and CD⁴ cell activation in the gut. J.Clin.Invest. 2002 PMID: 11927615
• Ma Prostaglandin E receptor EP₄ antagonism inhibits breast cancer metastasis. Cancer Res. 2006 PMID: 16540639