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Proteome Profiler Mouse Phospho-RTK Array Kit
R&D Systems, Inc. a Bio-Techne Brand
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Detection of Multiple Tyrosine Phosphorylated Receptors in Cell Lysates by the Mouse Phospho-RTK Array. The amount of lysate incubated with each array is indicated in the figure. Data shown are from 2 minute (Panels A, B, and C) or 5 minute (Panel D) exposure to X-ray film.A. M1 mouse myeloid leukemia cells were either untreated or treated with 500 ng/mL recombinant mouse Flt-3 Ligand (Catalog # 427-FL) for 5 minutes.B. NMuMG mouse mammary gland epithelial cells were either untreated or treated with 200 ng/mL recombinant mouse EGF (Catalog # 2028-EG) for 5 minutes.C. Hepa 1-6 mouse hepatoma cells were either untreated or treated with 1 µg/mL recombinant human insulin (Sigma, Catalog # I9278).D. HEK293 human embryonic kidney cells transfected with mouse EphA1 were either untreated or treated with 3 µg/mL mouse Ephrin-A1 (Catalog # 602-A1) and 0.3 µg/mL goat anti-human IgG Fc (Catalog # G-102-C) for 20 minutes. |
Citations for Proteome Profiler Mouse Phospho-RTK Array Kit (37)
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Citations are publications that use Bio-Techne products. Selected citations for Proteome Profiler Mouse Phospho-RTK Array Kit include:
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Species: N/A
Sample Types: Cell Lysates
G Egan et al. (2022), Impact of silk hydrogel secondary structure on hydrogel formation, silk leaching and in vitro response Scientific Reports, 12(1):3729.
PMID: 35260610 -
Species: Mouse
Sample Types: Cell Lysates
MB Kelley et al. (2022), Loss of Tpl2 activates compensatory signaling and resistance to EGFR/MET dual inhibition in v-RAS transduced keratinocytes PLoS ONE, 17(3):e0266017.
PMID: 35325006 -
Species: Mouse
Sample Types: Cell Lysates
S Hashiguchi et al. (2022), CCN2-induced lymphangiogenesis is mediated by the integrin alphavbeta5-ERK pathway and regulated by DUSP6 Scientific Reports, 12(1):926.
PMID: 35042954 -
Species: Mouse
Sample Types: Cell Supernates
S Song et al. (2022), EGFR/MET promotes hepatocellular carcinoma metastasis by stabilizing tumor cells and resisting to RTKs inhibitors in circulating tumor microemboli Cell Death Disease, 2022;13(4):351.
PMID: 35428350 -
Species: Mouse
Sample Types: Cell Lysates
CH Perry et al. (2022), MAP3K4 promotes fetal and placental growth by controlling the receptor tyrosine kinases IGF1R/IR and Akt signaling pathway� The Journal of Biological Chemistry, 0(0):102310.
PMID: 35921893 -
Species: Mouse
Sample Types: Protein Lysates
E Neto et al. (2022), Osteoclast-derived extracellular vesicles are implicated in sensory neurons sprouting through the activation of epidermal growth factor signaling Cell bioscience, 2022;12(1):127.
PMID: 35965312 -
Species: Mouse
Sample Types: Whole Cells
DA Kaji et al. (2021), Transcriptional profiling of mESC-derived tendon and fibrocartilage cell fate switch Nature Communications, 12(1):4208.
PMID: 34244516 -
Species: Mouse
Sample Types: Tissue Homogenates
CE Eyermann et al. (2021), The HSP-RTK-Akt axis mediates acquired resistance to Ganetespib in HER2-positive breast cancer Cell Death Disease, 2021;12(1):126.
PMID: 33500390 -
Species: Transgenic Mouse
Sample Types: Tissue Homogenates
H Niitsu et al. (2021), Cell-Autonomous Role of EGFR in Spontaneous Duodenal Tumors in LRIG1 Null Mice Cellular and Molecular Gastroenterology and Hepatology, 0(0).
PMID: 33989815 -
Species: Mouse
Sample Types: Cell Lysa
M Marqués et al. (2020), Combined MEK and PI3K/p110b inhibition as a novel targeted therapy for malignant mesothelioma displaying sarcomatoid features Cancer Res., 0(0).
PMID: 31911549 -
Species: Mouse
Sample Types: Cell Culture Lysates
N Yasuda et al. (2020), TAS-115 inhibits PDGFR&alpha/AXL/FLT-3 signaling and suppresses lung metastasis of osteosarcoma FEBS Open Bio, 0(0).
PMID: 32128992 -
Species: Mouse
Sample Types: Cell Lysates
B Lee et al. (2020), An autophagic deficit in the uterine vessel microenvironment provokes hyperpermeability through deregulated VEGFA, NOS1, and CTNNB1 Autophagy, 0(0):1-18.
PMID: 32579471 -
Species: Mouse
Sample Types: Tissue Homogenates
AS Ernst et al. (2019), EphB2-dependent signaling promotes neuronal excitotoxicity and inflammation in the acute phase of ischemic stroke Acta Neuropathol Commun, 7(1):15.
PMID: 30722785 -
Species: Mouse
Sample Types: Tissue Lysates
SL Roche et al. (2019), Norgestrel, a Progesterone Analogue, Promotes Significant Long-Term Neuroprotection of Cone Photoreceptors in a Mouse Model of Retinal Disease Invest. Ophthalmol. Vis. Sci., 60(8):3221-3235.
PMID: 31335948 -
Species: Mouse
Sample Types: Tissue Extracts
N Haq et al. (2019), Loss of Bardet-Biedl syndrome proteins causes synaptic aberrations in principal neurons PLoS Biol., 17(9):e3000414.
PMID: 31479441 -
Species: Mouse
Sample Types: Cell Culture Supernates
J Naipauer et al. (2019), PDGFRA defines the mesenchymal stem cell Kaposi's sarcoma progenitors by enabling KSHV oncogenesis in an angiogenic environment PLoS Pathog., 15(12):e1008221.
PMID: 31881074 -
Species: Mouse
Sample Types: Cell Lysates
R Imamaki et al. (2018), Glycosylation controls cooperative PECAM-VEGFR2-?3 integrin functions at the endothelial surface for tumor angiogenesis Oncogene, 0(0).
PMID: 29717262 -
Species: Mouse
Sample Types: Tissue Homogenates
A Yallowitz et al. (2018), Heat shock factor 1 confers resistance to lapatinib in ERBB2-positive breast cancer cells Cell Death Dis, 9(6):621.
PMID: 29799521 -
Species: Transfected
Sample Types: Cell Culture Supernates
LE Cavallin et al. (2018), KSHV-induced ligand mediated activation of PDGF receptor-alpha drives Kaposi's sarcomagenesis PLoS Pathog., 14(7):e1007175.
PMID: 29985958 -
Species: Mouse
Sample Types: Serum
Z D'Costa et al. (2017), Gemcitabine-induced TIMP1 attenuates therapy response and promotes tumor growth and liver metastasis in pancreatic cancer Cancer Res., 0(0).
PMID: 28765154 -
Species: Mouse
Sample Types: Cell Lysates
CH Shin et al. (2017), HBEGF promotes gliomagenesis in the context of Ink4a/Arf and Pten loss Oncogene, 0(0).
PMID: 28368403 -
Species: Mouse
Sample Types: Cell Lysates
DA Almiron Bo et al. (2017), Insulin-mediated signaling facilitates resistance to PDGFR inhibition in proneural hPDGFB-driven gliomas Mol. Cancer Ther, 0(0).
PMID: 28138037 -
Species: Mouse
Sample Types: Tissue Homogenates
M Ullrich et al. (2017), OCD-like behavior is caused by dysfunction of thalamo-amygdala circuits and upregulated TrkB/ERK-MAPK signaling as a result of SPRED2 deficiency Mol. Psychiatry, 0(0).
PMID: 28070119 -
Species: Mouse
Sample Types: Cell Lysates
D Zhang et al. (2017), Betacellulin regulates the proliferation and differentiation of retinal progenitor cells in vitro J. Cell. Mol. Med., 0(0).
PMID: 28922560 -
Species: Mouse
Sample Types: Tissue Homogenates
X Lu et al. (2017), Effective combinatorial immunotherapy for castration-resistant prostate cancer Nature, 543(7647):728-732.
PMID: 28321130 -
Species: Mouse
Sample Types: Tissue Homogenates
E Neto et al. (2017), Axonal outgrowth, neuropeptides expression and receptors tyrosine kinase phosphorylation in 3D organotypic cultures of adult dorsal root ganglia PLoS ONE, 12(7):e0181612.
PMID: 28742111 -
Species: Human
Sample Types: Cell Lysates
AK Piper et al. (2017), Enzymatic cleavage of myoferlin releases a dual C2-domain module linked to ERK signalling Cell. Signal, 33(0):30-40.
PMID: 28192161 -
Species: Mouse
Sample Types: Cell Lysates
Wang W et al. (2015), Epidermal growth factor receptor inhibition reduces angiogenesis via hypoxia-inducible factor-1alpha and Notch1 in head neck squamous cell carcinoma. PLoS ONE, 10(2):e0119723.
PMID: 25723392 -
Species: Mouse
Sample Types: Protein
Yang Y et al. (2014), ZEB1 sensitizes lung adenocarcinoma to metastasis suppression by PI3K antagonism. J Clin Invest, 124(6):2696-708.
PMID: 24762440 -
Species: Mouse
Sample Types: Cell Lysates
Stone S et al. (2014), HPV16-associated tumors control myeloid cell homeostasis in lymphoid organs, generating a suppressor environment for T cells. J Leukoc Biol, 96(4):619-31.
PMID: 24970861 -
Species: Mouse
Sample Types: Tissue Homogenates
Horvath Z et al. (2014), Decorin deficiency promotes hepatic carcinogenesis. Matrix Biol, 35(0):194-205.
PMID: 24361483 -
Species: Mouse
Sample Types: Tissue Homogenates
Fullar A et al. (2014), Lack of Matrilin-2 favors liver tumor development via Erk1/2 and GSK-3beta pathways in vivo. PLoS ONE, 9(4):e93469.
PMID: 24691449 -
Species: Mouse
Sample Types: Tissue Homogenates
Miyabayashi K et al. (2013), Erlotinib prolongs survival in pancreatic cancer by blocking gemcitabine-induced MAPK signals. Cancer Res, 73(7):2221-34.
PMID: 23378339 -
Species: Rat
Sample Types: Cell Lysates
Haines et al. (2012), Estrogenic compounds are not always cardioprotective and can be lethal in males with genetic heart disease. Endocrinology, 153(9):4470-9.
PMID: 22778230 -
Species: Mouse
Sample Types: Cell Lysates
Heckl D et al. (2012), Lentiviral vector induced insertional haploinsufficiency of Ebf1 causes murine leukemia. Mol. Ther., 20(6):1187-95.
PMID: 22472950 -
Species: Mouse
Sample Types: Tissue Homogenates
Chen J et al. (2012), Deletion of the epidermal growth factor receptor in renal proximal tubule epithelial cells delays recovery from acute kidney injury. Kidney Int., 82(1):45-52.
PMID: 22418982 -
Species: Mouse
Sample Types: Cell Lysates
Pickford CE et al. (2011), Specific glycosaminoglycans modulate neural specification of mouse embryonic stem cells. Stem Cells, 29(4):629-40.
PMID: 21308866
There are no citations that match your criteria.
Customer Reviews for Proteome Profiler Mouse Phospho-RTK Array Kit (7)
4.6 out of 5
7 customer ratings
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Verified Customer | Posted 10/16/2022Sample from FACS sorted cells, so limited amount of protein but still able to see some phospho RTK. Would definitely use again.
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Verified Customer | Posted 04/20/2019Array works and produces publishable results. Not happy with the pho-PDGFR antibody, sinse it appear to cross react with something in mouse tumor lysate
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Verified Customer | Posted 03/27/2019
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Verified Customer | Posted 04/16/2018
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Verified Customer | Posted 07/05/2017Lysates from mouse embryonic stem cells applied to membranes using instructions provided. Western blots were clean and worked great!
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Verified Customer | Posted 05/16/2017Worked really well on mouse tumour lysates.
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Verified Customer | Posted 06/29/2016Mammary tumors extracts from MMTV-Neu mice with different p53 genotypes (p53wt vs mutp53)
There are no reviews that match your criteria.
FAQs for Proteome Profiler Mouse Phospho-RTK Array Kit
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What are the phosphorylation site(s) of the 49 different tyrosine residues on the RTKs?
This kit uses a pan anti-phospho-tyrosine antibody as the detection antibody, which means it is capable of detecting phosphorylation at any available tyrosine. It is not designed to be specific for one phosphorylation site on each molecule. For instance, PDGF R alpha has multiple sites of tyrosine phosphorylation (see the “PTM/Processing” section of http://www.uniprot.org/uniprot/P16234 for a list of the currently identified sites).
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Can the Mouse Phospho-RTK Array be used for measuring the relative levels of total RTKs present in a sample?
No. Although the array kit uses capture antibodies that recognize both phosphorylated and unphosphorylated RTKs, the detection antibody is a pan anti-phospho-tyrosine antibody which only detects phosphorylated tyrosines on activated RTKs.
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To confirm positive signal for an RTK by IP-Western blot, are the capture antibodies from the Mouse Phospho-RTK Array Kit offered separately?
The identities of the capture antibodies on the Mouse Phospho-RTK Array are considered proprietary. However, to help investigators confirm their array results, Bio-Techne offers antibodies for each RTK detectable in the array kit. These antibodies are validated for Western blot and can be found in our catalog. A pan anti-phospho-tyrosine-HRP detection antibody is also available (Catalog # HAM1676).
