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Proteome Profiler Mouse Phospho-RTK Array Kit

Bio-Techne includes R&D Systems | Catalog # ARY014

Contains 4 membranes - each spotted in duplicate with 39 different RTK antibodies

Key Product Details

The Mouse Phospho-RTK Array detects multiple tyrosine phosphorylated receptors in untreated and ligand-treated cell lysates.

Citations for Proteome Profiler Mouse Phospho-RTK Array Kit (37)

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Customer Reviews for Proteome Profiler Mouse Phospho-RTK Array Kit (8)

4.5 out of 5
8 customer ratings
5 stars
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4 stars
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3 stars
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  • Proteome Profiler Mouse Phospho-RTK Array Kit
    Name: Anonymous
    Verified Customer | Posted 06/09/2023
    Use of RTK array to assay FACS sorted cells from murine lungs.
    Proteome Profiler Mouse Phospho-RTK Array Kit ARY014
  • Proteome Profiler Mouse Phospho-RTK Array Kit
    Name: Felipe Rodrigues
    Verified Customer | Posted 10/16/2022
    Sample from FACS sorted cells, so limited amount of protein but still able to see some phospho RTK. Would definitely use again.
    Proteome Profiler Mouse Phospho-RTK Array Kit ARY014
  • Proteome Profiler Mouse Phospho-RTK Array Kit
    Name: Anonymous
    Verified Customer | Posted 04/20/2019
    Array works and produces publishable results. Not happy with the pho-PDGFR antibody, sinse it appear to cross react with something in mouse tumor lysate
    Proteome Profiler Mouse Phospho-RTK Array Kit ARY014
  • Proteome Profiler Mouse Phospho-RTK Array Kit
    Name: Anonymous
    Verified Customer | Posted 03/27/2019
    Proteome Profiler Mouse Phospho-RTK Array Kit ARY014
  • Proteome Profiler Mouse Phospho-RTK Array Kit
    Name: Anonymous
    Verified Customer | Posted 04/16/2018
    Proteome Profiler Mouse Phospho-RTK Array Kit ARY014
  • Proteome Profiler Mouse Phospho-RTK Array Kit
    Name: Mia Huang
    Verified Customer | Posted 07/05/2017
    Lysates from mouse embryonic stem cells applied to membranes using instructions provided. Western blots were clean and worked great!
    Proteome Profiler Mouse Phospho-RTK Array Kit ARY014
  • Proteome Profiler Mouse Phospho-RTK Array Kit
    Name: Natasha Tracey
    Verified Customer | Posted 05/16/2017
    Worked really well on mouse tumour lysates.
    Proteome Profiler Mouse Phospho-RTK Array Kit ARY014
  • Proteome Profiler Mouse Phospho-RTK Array Kit
    Name: Natalia Marchenko
    Verified Customer | Posted 06/29/2016
    Mammary tumors extracts from MMTV-Neu mice with different p53 genotypes (p53wt vs mutp53)
    Proteome Profiler Mouse Phospho-RTK Array Kit ARY014

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FAQs for Proteome Profiler Mouse Phospho-RTK Array Kit

  • What are the phosphorylation site(s) of the 49 different tyrosine residues on the RTKs?

    This kit uses a pan anti-phospho-tyrosine antibody as the detection antibody, which means it is capable of detecting phosphorylation at any available tyrosine. It is not designed to be specific for one phosphorylation site on each molecule. For instance, PDGF R alpha has multiple sites of tyrosine phosphorylation (see the “PTM/Processing” section of http://www.uniprot.org/uniprot/P16234 for a list of the currently identified sites). 

  • Can the Mouse Phospho-RTK Array be used for measuring the relative levels of total RTKs present in a sample?

    No. Although the array kit uses capture antibodies that recognize both phosphorylated and unphosphorylated RTKs, the detection antibody is a pan anti-phospho-tyrosine antibody which only detects phosphorylated tyrosines on activated RTKs.

  • To confirm positive signal for an RTK by IP-Western blot, are the capture antibodies from the Mouse Phospho-RTK Array Kit offered separately?

    The identities of the capture antibodies on the Mouse Phospho-RTK Array are considered proprietary. However, to help investigators confirm their array results, Bio-Techne offers antibodies for each RTK detectable in the array kit. These antibodies are validated for Western blot and can be found in our catalog. A pan anti-phospho-tyrosine-HRP detection antibody is also available (Catalog # HAM1676).

Product Documents for Proteome Profiler Mouse Phospho-RTK Array Kit

Certificate of Analysis

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