Does ARY025 detect free protease/protease inhibitor or protease/protease inhibitor complexes?

We have confirmed ARY025 reacts with free protease and protease inhibitor. We have not evaluated protease complexes and protease inhibitor complexes in this array.

Could you tell us whether this MMP-9 antibody can detect TIMP and MMP-9 complex?

We haven't performed a large amount of specificity testing per each spot in the arrays because these are qualitative screening tools. However, we would recommend treating the MMP-9 spot as a "Total MMP-9" detecting both free MMP-9 and MMP-9 in complex. If the MMP-9 spot turns out to be of high interest for the customer's study, they will want to examine this target in more detail with quantitative tools such as ELISA or Luminex.

Proteome Profiler Human Protease/Protease Inhibitor Array

Name: Proteome Profiler Human Protease/Protease Inhibitor Array
Brand: R&D Systems
SKU: ARY025

R&D Systems, Inc. a Bio-Techne Brand

Catalog # ARY025

Citations (7)

Product Datasheet / COA / SDS

Contains 4 membranes-2 spotted in duplicate with antibodies against 35 different proteases and 2 spotted in duplicate with antibodies against 32 different protease inhibitors

Key Product Details

Catalog #

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Setting the standard in quality research reagents

Technical Data
Citations (7) / Reviews / FAQs

Summary for Proteome Profiler Human Protease/Protease Inhibitor Array

Kit Summary

To simultaneously detect the relative changes of 32 protease inhibiting analytes in a single sample. No specialized equipment is necessary.

General Assay Principle

Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernates, cell and tissue lysates, serum, plasma, urine, saliva, or human milk samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any protein/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of protein bound. Chemiluminescence is detected in the same manner as a Western blot.

Kit Contents

4 Array Membranes (2 of Proteases and 2 Protease Inhibitors)
4-Well Multi-dish
Array Buffers
Wash Buffer
Detection Antibody Cocktail, Human Protease Array
Detection Antibody Cocktail, Human Protease Inhibitor Array
Streptavidin-HRP
Chemiluminescent Detection Reagents
Transparency Overlay Template
Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Simultaneously detect the levels of these protease inhibitor proteins in a single sample.
APP	Lipocalin-1	Serpin F1/PEDF
Cystatin A	Lipocalin-2/NGAL	Testican 1/SPOCK1
Cystatin B	RECK	Testican 2/SPOCK2
Cystatin C	Serpin A5	TFPI
Cystatin E/M	Serpin A8/AGT	TFPI-2
EMMPRIN	Serpin A9/Centerin	TIMP-1
Fetuin B	Serpin A12	TIMP-2
HAI-1	Serpin B5/Maspin	TIMP-3
HAI-2	Serpin B6	TIMP-4
HE4/WFDC2	Serpin B8	Trappin-2/Elafin
Latexin	Serpin E1/PAI-1

Simultaneously detect the levels of these proteases in a single sample.
ADAM8	Cathepsin X/Z/P	MMP-7
ADAM9	DPPIV/CD26	MMP-8
ADAMTS1	Kallikrein 3/PSA	MMP-9
ADAMTS13	Kallikrein 5	MMP-10
Cathepsin A	Kallikrein 6	MMP-12
Cathepsin B	Kallikrein 7	MMP-13
Cathepsin C/DPPI	Kallikrein 10	Neprilysin/CD10
Cathepsin D	Kallikrein 11	Presenilin-1
Cathepsin E	Kallikrein 13	Proprotein Convertase 9
Cathepsin L	MMP-1	Proteinase 3
Cathepsin S	MMP-2	uPA/Urokinase
Cathepsin V	MMP-3

Product Specifications for Proteome Profiler Human Protease/Protease Inhibitor Array

Species

Human

Preparation & Storage

Shipping Conditions	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Citations for Proteome Profiler Human Protease/Protease Inhibitor Array (7)

Showing 1 - 7 of 7 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Proteome Profiler Human Protease/Protease Inhibitor Array include:

Species: Human
Sample Types: Cell Culture Supernates

L Cook et al. (2022), ADAM8-Dependent Extracellular Signaling in the Tumor Microenvironment Involves Regulated Release of Lipocalin 2 and MMP-9 International Journal of Molecular Sciences, 23(4).
PMID: 35216088
Species: Mouse
Sample Types: Tissue Lysates

Y Xiao et al. (2022), Copper promotes cardiac functional recovery via suppressing the transformation of fibroblasts to myofibroblasts in ischemia-infarcted monkey hearts The Journal of nutritional biochemistry, 0(0):109180.
PMID: 36240958
Species: Human
Sample Types: Cell Lysates

S Herbster et al. (2021), Low RECK Expression Is Part of the Cervical Carcinogenesis Mechanisms Cancers, 13(9).
PMID: 34066355
Species: Human
Sample Types: Whole Cells
Applications: Protein Array

BT Elie et al. (2018), A heterometallic ruthenium-gold complex displays antiproliferative, antimigratory, and antiangiogenic properties and inhibits metastasis and angiogenesis-associated proteases in renal cancer J. Biol. Inorg. Chem., 0(0).
PMID: 29508136
Species: Human
Sample Types: Cell Culture Supernates

T Nargis et al. (2017), KLK5 induces shedding of DPP4 from circulatory Th17 cells in type 2 diabetes Mol Metab, 6(11):1529-1539.
PMID: 29107298
Species: Human
Sample Types: Cell Lysates

J Kryczka et al. (2017), Proteases Revisited: Roles and Therapeutic Implications in Fibrosis Mediators Inflamm., 2017(0):2570154.
PMID: 28642633
Species: Human
Sample Types: Cell Culture Supernates

J Lee et al. (2017), Use of ethanol extracts of Terminalia chebula to prevent periodontal disease induced by dental plaque bacteria BMC Complement Altern Med, 17(1):113.
PMID: 28202081

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Showing 1 - 7 of 7 citations Showing All

FAQs for Proteome Profiler Human Protease/Protease Inhibitor Array

Does ARY025 detect free protease/protease inhibitor or protease/protease inhibitor complexes?

We have confirmed ARY025 reacts with free protease and protease inhibitor. We have not evaluated protease complexes and protease inhibitor complexes in this array.
Could you tell us whether this MMP-9 antibody can detect TIMP and MMP-9 complex?

We haven't performed a large amount of specificity testing per each spot in the arrays because these are qualitative screening tools. However, we would recommend treating the MMP-9 spot as a "Total MMP-9" detecting both free MMP-9 and MMP-9 in complex. If the MMP-9 spot turns out to be of high interest for the customer's study, they will want to examine this target in more detail with quantitative tools such as ELISA or Luminex.

Proteome Profiler Human Protease/Protease Inhibitor Array

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Summary for Proteome Profiler Human Protease/Protease Inhibitor Array

General Assay Principle

Product Specifications for Proteome Profiler Human Protease/Protease Inhibitor Array

Preparation & Storage

Citations for Proteome Profiler Human Protease/Protease Inhibitor Array (7)

FAQs for Proteome Profiler Human Protease/Protease Inhibitor Array

Product Documents for Proteome Profiler Human Protease/Protease Inhibitor Array

Product Datasheets

COA

Certificate of Analysis

SDS

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