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Zyxin Antibody (CL2502) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44466

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NBP3-44466
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL2502

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of Q15942, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Zyxin Antibody (CL2502) - Azide and BSA Free

Zyxin Antibody (CL2502) - Azide and BSA Free Immunohistochemistry-Paraffin: Zyxin Antibody [NBP3-44466]

Immunohistochemistry-Paraffin: Zyxin Antibody [NBP3-44466]

Staining of human cerebral cortex shows no positivity in neuropil as expected.
Zyxin Antibody (CL2502) - Azide and BSA Free Immunohistochemistry-Paraffin: Zyxin Antibody [NBP3-44466]

Immunohistochemistry-Paraffin: Zyxin Antibody [NBP3-44466]

Staining of human endometrium shows strong cytoplasmic positivity in stromal cells.
Zyxin Antibody (CL2502) - Azide and BSA Free Immunohistochemistry-Paraffin: Zyxin Antibody [NBP3-44466]

Immunohistochemistry-Paraffin: Zyxin Antibody [NBP3-44466]

Staining of human kidney shows moderate to strong cytoplasmic and membranous positivity in cells in glomeruli and in tubules.

Applications for Zyxin Antibody (CL2502) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Zyxin

Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform.

Alternate Names

Zyx, Zyxin 2

Gene Symbol

ZYX

Additional Zyxin Products

Product Documents for Zyxin Antibody (CL2502) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Zyxin Antibody (CL2502) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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