ZNRF3 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-03924
![Immunohistochemistry-Paraffin: ZNRF3 Antibody - Azide and BSA Free [NBP3-03924]](https://resources.bio-techne.com/images/products/ZNRF3-Antibody-Immunohistochemistry-Paraffin-NBP3-03924-img0004.jpg "Immunohistochemistry-Paraffin: ZNRF3 Antibody - Azide and BSA Free [NBP3-03924]")
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human ZNRF3 (NP_001193927.1). MHPLGLCNNNDEEDLYEYGWVGVVKLEQPELDPKPCLTVLGKAKRAVQRGATAVIFDVSENPEAIDQLNQGSEDPLKRPVVYVKGADAIKLMNIVNKQKVA
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit ZNRF3 Antibody - BSA Free (NBP3-03924) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-ZNRF3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for ZNRF3 Antibody - BSA Free
![Western Blot: ZNRF3 AntibodyAzide and BSA Free [NBP3-03924]](https://resources.bio-techne.com/images/products/ZNRF3-Antibody-Western-Blot-NBP3-03924-img0006.jpg "Western Blot: ZNRF3 AntibodyAzide and BSA Free [NBP3-03924]")
Western Blot: ZNRF3 AntibodyAzide and BSA Free [NBP3-03924]
Western Blot: ZNRF3 Antibody [NBP3-03924] - Analysis of extracts of Mouse testis, using ZNRF3 antibody at 1:3416 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.![Immunohistochemistry-Paraffin: ZNRF3 Antibody - Azide and BSA Free [NBP3-03924]](https://resources.bio-techne.com/images/products/ZNRF3-Antibody-Immunohistochemistry-Paraffin-NBP3-03924-img0005.jpg "Immunohistochemistry-Paraffin: ZNRF3 Antibody - Azide and BSA Free [NBP3-03924]")
Immunohistochemistry-Paraffin: ZNRF3 Antibody - Azide and BSA Free [NBP3-03924]
Immunohistochemistry-Paraffin: ZNRF3 Antibody [NBP3-03924] - Rat lung using ZNRF3 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.![Immunohistochemistry-Paraffin: ZNRF3 Antibody - Azide and BSA Free [NBP3-03924]](https://resources.bio-techne.com/images/products/ZNRF3-Antibody-Immunohistochemistry-Paraffin-NBP3-03924-img0003.jpg "Immunohistochemistry-Paraffin: ZNRF3 Antibody - Azide and BSA Free [NBP3-03924]")
Immunohistochemistry-Paraffin: ZNRF3 Antibody - Azide and BSA Free [NBP3-03924]
Immunohistochemistry-Paraffin: ZNRF3 Antibody [NBP3-03924] - Human liver using ZNRF3 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for ZNRF3 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:1000 - 1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ZNRF3
The cell-surface transmembrane E3 ubiquitin ligase zinc and ring finger 3 (ZNRF3) and its homologue ring finger 43 (RNF43) are negative feedback regulators of Wnt signaling. ZNRF3 is associated with the Wnt receptor complex, and inhibits Wnt signaling by promoting the turnover of frizzled and LRP6. R-spondin may enhance Wnt signaling by inhibiting ZNRF3. ZNRF3 may be a target for therapeutic exploration.
Long Name
Zinc and Ring Finger 3
Alternate Names
BK747E2.3, KIAA1133, RNF203
Gene Symbol
ZNRF3
Additional ZNRF3 Products
Product Documents for ZNRF3 Antibody - BSA Free
Product Specific Notices for ZNRF3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...