ZNF518B Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81380
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ATPVLIPKGAVLRVLNSSENAHIIEATCEAPVSIPCSERQLIKPVPFCPVRQADSDLQPLRSERGPIDMSPNIETPLRPKLRKESAVCSTIH
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ZNF518B Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: ZNF518B Antibody [NBP1-81380]](https://resources.bio-techne.com/images/products/ZNF518B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81380-img0004.jpg "Immunocytochemistry/ Immunofluorescence: ZNF518B Antibody [NBP1-81380]")
Immunocytochemistry/ Immunofluorescence: ZNF518B Antibody [NBP1-81380]
Immunocytochemistry/Immunofluorescence: ZNF518B Antibody [NBP1-81380] - Staining of human cell line U-2 OS shows localization to nucleus. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: ZNF518B Antibody [NBP1-81380]](https://resources.bio-techne.com/images/products/ZNF518B-Antibody-Immunohistochemistry-Paraffin-NBP1-81380-img0002.jpg "Immunohistochemistry-Paraffin: ZNF518B Antibody [NBP1-81380]")
Immunohistochemistry-Paraffin: ZNF518B Antibody [NBP1-81380]
Immunohistochemistry-Paraffin: ZNF518B Antibody [NBP1-81380] - Staining of human kidney shows strong nuclear positivity in renal tubules.
Chromatin Immunoprecipitation ChIP: ZNF518B Antibody - BSA Free
ChIP-Exo-Seq composite graph for Anti-ZNF518B tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh's Lab at Cornell University.Applications for ZNF518B Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation-exo-Seq
1-10ug per reaction
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ZNF518B
Alternate Names
zinc finger protein 518B
Gene Symbol
ZNF518B
Additional ZNF518B Products
Product Documents for ZNF518B Antibody - BSA Free
Product Specific Notices for ZNF518B Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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