ZNF445 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80598
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq
Cited:
Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: QPKGNPVAAPTGDDLQSKTNKFILNQEPLEEAETLAVSSGCPATSVSEGIGLRESFQQKSRQKDQCENPIQVRVKKEETNFSHRTGKDSEVSGSNSLDLKHVTYLRVSGRKESLKHGCGKHFRMSSHHYDYKKYGK
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit ZNF445 Antibody - BSA Free (NBP1-80598) is a polyclonal antibody validated for use in IHC and ICC/IF. Anti-ZNF445 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for ZNF445 Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: ZNF445 Antibody [NBP1-80598]](https://resources.bio-techne.com/images/products/ZNF445-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80598-img0005.jpg "Immunocytochemistry/ Immunofluorescence: ZNF445 Antibody [NBP1-80598]")
Immunocytochemistry/ Immunofluorescence: ZNF445 Antibody [NBP1-80598]
Immunocytochemistry/Immunofluorescence: ZNF445 Antibody [NBP1-80598] - Immunofluorescent staining of human cell line SiHa shows localization to nucleus, cytosol & mitochondria.![Immunohistochemistry-Paraffin: ZNF445 Antibody [NBP1-80598]](https://resources.bio-techne.com/images/products/ZNF445-Antibody-Immunohistochemistry-Paraffin-NBP1-80598-img0006.jpg "Immunohistochemistry-Paraffin: ZNF445 Antibody [NBP1-80598]")
Immunohistochemistry-Paraffin: ZNF445 Antibody [NBP1-80598]
Immunohistochemistry-Paraffin: ZNF445 Antibody [NBP1-80598] - Staining of human kidney shows strong granular cytoplasmic positivity in tubule cells.![ZNF445 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: ZNF445 Antibody - BSA Free [NBP1-80598]](https://resources.bio-techne.com/images/products/nbp1-80598_rabbit-polyclonal-znf445-antibody-25620259121924.jpg "Chromatin Immunoprecipitation-exo-Seq: ZNF445 Antibody - BSA Free [NBP1-80598]")
Chromatin Immunoprecipitation-exo-Seq: ZNF445 Antibody - BSA Free [NBP1-80598]
ChIP-Exo-Seq composite graph for Anti-ZNF445 (NBP1-80598) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.Applications for ZNF445 Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation-exo-Seq
1-10ug per reaction
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ZNF445
Alternate Names
zinc finger protein 168, zinc finger protein 445, zinc finger protein with KRAB and SCAN domains 15, ZKSCAN15, ZNF168
Entrez Gene IDs
353274 (Human)
Gene Symbol
ZNF445
UniProt
Additional ZNF445 Products
Product Documents for ZNF445 Antibody - BSA Free
Product Specific Notices for ZNF445 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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