ZNF434 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82182
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: GEEAVALVEDVQRAPGQQVLDSEKDLKVLMKEMAPLGATRESLRSQWKQEVQPEEPTFKGSQSSHQRPGEQSEAWLAPQAPRNLPQNTGLH
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ZNF434 Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: ZNF434 Antibody [NBP1-82182]](https://resources.bio-techne.com/images/products/ZNF434-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82182-img0005.jpg "Immunocytochemistry/ Immunofluorescence: ZNF434 Antibody [NBP1-82182]")
Immunocytochemistry/ Immunofluorescence: ZNF434 Antibody [NBP1-82182]
Immunocytochemistry/Immunofluorescence: ZNF434 Antibody [NBP1-82182] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.![Immunohistochemistry-Paraffin: ZNF434 Antibody [NBP1-82182]](https://resources.bio-techne.com/images/products/ZNF434-Antibody-Immunohistochemistry-Paraffin-NBP1-82182-img0006.jpg "Immunohistochemistry-Paraffin: ZNF434 Antibody [NBP1-82182]")
Immunohistochemistry-Paraffin: ZNF434 Antibody [NBP1-82182]
Immunohistochemistry-Paraffin: ZNF434 Antibody [NBP1-82182] - Staining of human tonsil shows strong cytoplasmic positivity in lymphoid cells outside reaction centra.
Chromatin Immunoprecipitation ChIP: ZNF434 Antibody - BSA Free
ChIP-Exo-Seq composite graph for Anti-ZNF434 tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh's Lab at Cornell University.Applications for ZNF434 Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation-exo-Seq
1-10ug per reaction
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ZNF434
Additional ZNF434 Products
Product Documents for ZNF434 Antibody - BSA Free
Product Specific Notices for ZNF434 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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