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ZNF295 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-74655

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 875 and 925 of human zinc finger protein 295 using the numbering given in entry NP_065778.3 (GeneID 49854).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZNF295 Antibody - BSA Free

Western Blot: ZNF295 Antibody [NB100-74655]

Western Blot: ZNF295 Antibody [NB100-74655]

Western Blot: ZNF295 Antibody [NB100-74655] - Detection of Human ZNF295 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ZNF295 antibody NB100-74655 used for IP at 3 mcg/mg lysate. ZNF295 was also immunoprecipitated by rabbit anti-ZNF295 antibody NB100-74656, which recognizes a downstream epitope.
Immunocytochemistry/ Immunofluorescence: ZNF295 Antibody [NB100-74655]

Immunocytochemistry/ Immunofluorescence: ZNF295 Antibody [NB100-74655]

Immunocytochemistry/Immunofluorescence: ZNF295 Antibody [NB100-74655] - Detection: DAB and Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight 594 Conjugated used at a dilution of 1:100
Immunohistochemistry: ZNF295 Antibody [NB100-74655]

Immunohistochemistry: ZNF295 Antibody [NB100-74655]

Immunohistochemistry: ZNF295 Antibody [NB100-74655] - Sample: FFPE sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-ZNF295 used at a dilution of 1:1,000 (1ug/ml) and 1:400 (2.5ug/ml).

Applications for ZNF295 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:250-1:1000

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

2-5ug/mg Lysate
Application Notes
This antibody is not recommended for Western Blot analysis.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ZNF295

ZNF295 bears 8 C2H2-type zinc fingers and a BTB/POZ (broad-complex, tramtrack, bric a brac/ Pox-virus and zinc finger) domain indicating a function in transcriptional regulation. ZNF295 has been shown to function as a transcriptional repressor and associates with ZFP161, a zinc-finger protein also know to function as a repressor of transcription.

Alternate Names

MGC15716, ZFP414, zinc finger protein 414

Entrez Gene IDs

49854 (Human)

Gene Symbol

ZNF295

Additional ZNF295 Products

Product Documents for ZNF295 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ZNF295 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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