ZNF277 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82529
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Rat (93%). Backed by our 100% Guarantee.
Applications
Chromatin Immunoprecipitation-exo-Seq, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: FPVAEQDKLLKHMIIEHKIVIADVKLVADFQRYILYWRKRFTEQPITDFCSVIRINSTAPFEEQENYFLLCDVLPEDRILREELQKQRLREILEQQQQERNDTNFHGVCMFCNEEFLGNRSVILNH
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit ZNF277 Antibody - BSA Free (NBP1-82529) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for ZNF277 Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: ZNF277 Antibody [NBP1-82529]](https://resources.bio-techne.com/images/products/ZNF277-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82529-img0005.jpg "Immunocytochemistry/ Immunofluorescence: ZNF277 Antibody [NBP1-82529]")
Immunocytochemistry/ Immunofluorescence: ZNF277 Antibody [NBP1-82529]
Immunocytochemistry/Immunofluorescence: ZNF277 Antibody [NBP1-82529] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.![Immunohistochemistry-Paraffin: ZNF277 Antibody [NBP1-82529]](https://resources.bio-techne.com/images/products/ZNF277-Antibody-Immunohistochemistry-Paraffin-NBP1-82529-img0006.jpg "Immunohistochemistry-Paraffin: ZNF277 Antibody [NBP1-82529]")
Immunohistochemistry-Paraffin: ZNF277 Antibody [NBP1-82529]
Immunohistochemistry-Paraffin: ZNF277 Antibody [NBP1-82529] - Staining of human lymph node shows strong nuclear staining in reaction center cells while subsets of cells outside the reaction center displayed strong positivity the cytoplasm.![ZNF277 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: ZNF277 Antibody - BSA Free [NBP1-82529]](https://resources.bio-techne.com/images/products/nbp1-82529_rabbit-polyclonal-znf277-antibody-256202591169.jpg "Chromatin Immunoprecipitation-exo-Seq: ZNF277 Antibody - BSA Free [NBP1-82529]")
Chromatin Immunoprecipitation-exo-Seq: ZNF277 Antibody - BSA Free [NBP1-82529]
ChIP-Exo-Seq composite graph for Anti-ZNF277 (NBP1-82529) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.
Applications for ZNF277 Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation-exo-Seq
1-10µg per reaction
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ZNF277
Alternate Names
FLJ40981, zinc finger protein 433
Gene Symbol
ZNF277
Additional ZNF277 Products
Product Documents for ZNF277 Antibody - BSA Free
Product Specific Notices for ZNF277 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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