ZNF276 Antibody - BSA Free

Immunohistochemistry: ZNF276 Antibody - BSA Free [NBP1-82681] -

ZNF276 activates Wnt/ beta-catenin signaling through recruitment of MAGEB2 to regulate CYP1B1 transcription.A GO analysis of the potential ZNF276-interacted proteins. B KEGG analysis of the potential ZNF276-interacted proteins. C, D Both forward (C) and reverse (D) co-IP analysis affirmed the interaction between ZNF276 and MAGEB2. E ChIP-PCR analysis of ZNF276 binding ability at the CYP1B1 promoter affected by MAGEB2 overexpression. F, G The luciferase activity of CYP1B1 promoter decreased by ZNF276 silencing was further enhanced by the expression of MAGEB2 in 293T and MCF-7. H, I RT-qPCR (H) and western blot (I) analysis confirmed the effect of MAGEB2 overexpression in CYP1B1 mRNA and protein expression in MCF-7 cells. J, K The reduced transcriptional activity of CYP1B1 promoter by ZNF276 was partially reversed by MAGEB2. L, M Overexpression of MAGEB2 reversed the decreased cell proliferation, migration and invasion abilities affected by ZNF276 silencing in CCK-8 assay (L), transwell migration (M, up) and transwell invasion (M, down). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36085146), licensed under a CC-BY license. Not internally tested by Novus Biologicals.