Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all ZHX1 Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residues 1-50 of human zinc fingers and homeoboxes 1 using the TrEMBL entry Q9UKY1 (GeneID 11244).

Reactivity Notes

Chimpanzee (100%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZHX1 Antibody - BSA Free

Western Blot: ZHX1 Antibody [NB600-244]

Western Blot: ZHX1 Antibody [NB600-244]

Western Blot: ZHX1 Antibody [NB600-244] - Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-ZHX1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: ZHX1 Antibody [NB600-244]

Immunohistochemistry-Paraffin: ZHX1 Antibody [NB600-244]

Immunohistochemistry-Paraffin: ZHX1 Antibody [NB600-244] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti- ZHX1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: ZHX1 Antibody [NB600-244]

Immunoprecipitation: ZHX1 Antibody [NB600-244]

Immunoprecipitation: ZHX1 Antibody [NB600-244] - Detection of human ZHX1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZHX1 antibody NB600-244 used for IP at 3 ug per reaction. ZHX1 was also immunoprecipitated by another rabbit anti-ZHX1 antibody. For blotting immunoprecipitated ZHX1, NB600-244 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Applications for ZHX1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ZHX1

Zinc-fingers and homeoboxes 1 (ZHX1) is a protein which interacts with the activation domain of the A subunit of nuclear factor-Y. It is a transcriptional repressor protein.

Alternate Names

zona pellucida B protein, zona pellucida glycoprotein 4ZBP, Zona pellucida protein B, zona pellucida sperm-binding protein 4, Zp-4, ZPBZP1

Entrez Gene IDs

11244 (Human)

Gene Symbol

ZHX1

UniProt

Q9UKY1 (Human)

Additional ZHX1 Products

Product Documents for ZHX1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ZHX1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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