ZFYVE16 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36538
![Western Blot: ZFYVE16 Antibody [NBP2-36538]](https://resources.bio-techne.com/images/products/ZFYVE16-Antibody-Western-Blot-NBP2-36538-img0001.jpg "Western Blot: ZFYVE16 Antibody [NBP2-36538]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by the antibody maps to a region between residue 450 to 500 of human Endosome-Associated FYVE Domain Protein using the numbering given in entry NP_001098721.1.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit ZFYVE16 Antibody - BSA Free (NBP2-36538) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for ZFYVE16 Antibody - BSA Free
Western Blot: ZFYVE16 Antibody [NBP2-36538]
Western Blot: ZFYVE16 Antibody [NBP2-36538] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Endofin antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.![Immunoprecipitation: ZFYVE16 Antibody [NBP2-36538]](https://resources.bio-techne.com/images/products/ZFYVE16-Antibody-Immunoprecipitation-NBP2-36538-img0002.jpg "Immunoprecipitation: ZFYVE16 Antibody [NBP2-36538]")
Immunoprecipitation: ZFYVE16 Antibody [NBP2-36538]
Immunoprecipitation: ZFYVE16 Antibody [NBP2-36538] - Detection of human Endofin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Endofin antibody NBP2-36538) used for IP at 6 ug per reaction. Endofin was also immunoprecipitated by rabbit anti-Endofin antibody NBP2-36539. For blotting immunoprecipitated Endofin, NBP2-36538 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.Applications for ZFYVE16 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2 - 10 ug/mg
Western Blot
1:2000 - 1:10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Validation by IP/Western Blot was performed using a 4-8% SDS-PAGE gel.
Validation by IP/Western Blot was performed using a 4-8% SDS-PAGE gel.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: ZFYVE16
Alternate Names
DKFZp686E13162, Endofin, endosome-associated FYVE-domain protein, KIAA0305Endosome-associated FYVE domain protein, zinc finger FYVE domain-containing protein 16, zinc finger, FYVE domain containing 16
Gene Symbol
ZFYVE16
UniProt
Additional ZFYVE16 Products
Product Documents for ZFYVE16 Antibody - BSA Free
Product Specific Notices for ZFYVE16 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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