ZCCHC8 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-94995

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated

Cited:

Western Blot, Immunoprecipitation, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all ZCCHC8 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human zinc finger, CCHC domain containing 8 using the numbering given in entry NP_060082.2 (GeneID 55596).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit ZCCHC8 Antibody - BSA Free (NB100-94995) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-ZCCHC8 Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ZCCHC8 Antibody - BSA Free

Western Blot: ZCCHC8 Antibody [NB100-94995]

Western Blot: ZCCHC8 Antibody [NB100-94995]

Western Blot: ZCCHC8 Antibody [NB100-94995] - Analysis of ZCCHC8 in HeLa whole cell lsyate using anti-ZCCHC8 antibody. Lane1: Control siRNA , Lane 2: HeLa cells treated with ZCCHC8 siRNA . Image from verified customer review.
Immunohistochemistry-Paraffin: ZCCHC8 Antibody [NB100-94995]

Immunohistochemistry-Paraffin: ZCCHC8 Antibody [NB100-94995]

Immunohistochemistry-Paraffin: ZCCHC8 Antibody [NB100-94995] - Sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-used at a dilution of 1:5,000 (0.2ug/ml) and 1:2,000 (0.5ug/ml). Detection: DAB and Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain, cross-adsorbedAntibody DyLight (R) 594 Conjugated used at a dilution of 1:100
Immunoprecipitation: ZCCHC8 Antibody [NB100-94995]

Immunoprecipitation: ZCCHC8 Antibody [NB100-94995]

Immunoprecipitation: ZCCHC8 Antibody [NB100-94995] - Detection of Human ZCCHC8 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-ZCCHC8 antibody NB100-94995 used for IP at 3 mcg/mg lysate. ZCCHC8 was also immunoprecipitated by rabbit anti-ZCCHC8 antibody NB100-94996, which recognizes a downstream epitope.

Applications for ZCCHC8 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000 - 1:5000

Immunohistochemistry

1:2000 - 1:10000

Immunohistochemistry-Paraffin

1:2000 - 1:10000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:100-1:2000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. ZCCHC8 antibody validated for WB from a verified customer review.

Reviewed Applications

Read 2 reviews rated 5 using NB100-94995 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ZCCHC8

ZCCHC8 is a zinc-finger protein that bears a zinc-binding motif composed of a CX2CX4HX4C sequence (where X can be any amino acid). ZCCHC8 also bears a PSP domain, a proline rich domain found in numerous splicesome associated proteins. ZCCHC8 has been identified as a component of the splicesome C complex and as a substrate of glycogen synthase kinase-3. It is suggested to play a role in the processing and metabolism of RNA.

Alternate Names

DKFZp434E2220, zinc finger CCHC domain-containing protein 8, zinc finger, CCHC domain containing 8

Entrez Gene IDs

55596 (Human)

Gene Symbol

ZCCHC8

UniProt

NP_060082.2 (Human)

Additional ZCCHC8 Products

Product Documents for ZCCHC8 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ZCCHC8 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.