ZBTB25 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-31583
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: IGLDDGTADQQRACPATQALEEHQKPPVSIKQERCDPESVISQSHPSPSSEVTGPTFTENSVKIHLCHYCGERFDSRSNLR
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (86%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit ZBTB25 Antibody - BSA Free (NBP2-31583) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for ZBTB25 Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: ZBTB25 Antibody [NBP2-31583]](https://resources.bio-techne.com/images/products/ZBTB25-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-31583-img0005.jpg "Immunocytochemistry/ Immunofluorescence: ZBTB25 Antibody [NBP2-31583]")
Immunocytochemistry/ Immunofluorescence: ZBTB25 Antibody [NBP2-31583]
Immunocytochemistry/Immunofluorescence: ZBTB25 Antibody [NBP2-31583] - Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.![Immunohistochemistry: ZBTB25 Antibody [NBP2-31583]](https://resources.bio-techne.com/images/products/ZBTB25-Antibody-Immunohistochemistry-NBP2-31583-img0002.jpg "Immunohistochemistry: ZBTB25 Antibody [NBP2-31583]")
Immunohistochemistry: ZBTB25 Antibody [NBP2-31583]
Immunohistochemistry: ZBTB25 Antibody [NBP2-31583] - Staining of human testis shows strong nuclear positivity in subset of cells in seminiferus ducts.
Chromatin Immunoprecipitation ChIP: ZBTB25 Antibody - BSA Free
ChIP-Exo-Seq composite graph for Anti-ZBTB25 tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh's Lab at Cornell University.Applications for ZBTB25 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ZBTB25
Alternate Names
C14orf51, KUPchromosome 14 open reading frame 51, zinc finger and BTB domain containing 25, zinc finger and BTB domain-containing protein 25, Zinc finger protein 46, Zinc finger protein KUP, ZNF46zinc finger protein 46 (KUP)
Gene Symbol
ZBTB25
UniProt
Additional ZBTB25 Products
Product Documents for ZBTB25 Antibody - BSA Free
Product Specific Notices for ZBTB25 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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