Wnt-5a Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24752
![Wnt-5a Antibody - BSA Free Immunohistochemistry-Paraffin: Wnt-5a Antibody - BSA Free [NBP2-24752]](https://resources.bio-techne.com/images/products/antibody/nbp2-24752_rabbit-polyclonal-wnt-5a-antibody-imgenex-img-6075a-immunohistochemistry-paraffin-162202682014.png "Immunohistochemistry-Paraffin: Wnt-5a Antibody - BSA Free [NBP2-24752]")
![Western Blot: Wnt-5a AntibodyBSA Free [NBP2-24752]](https://resources.bio-techne.com/images/products/Wnt-5a-Antibody-Western-Blot-NBP2-24752-img0004.jpg "Western Blot: Wnt-5a AntibodyBSA Free [NBP2-24752]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Bovine, Monkey
Cited:
Human, Mouse
Predicted:
Amphibian (90%), Guinea Pig (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot
Cited:
Immunohistochemistry-Paraffin, Western Blot, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A synthetic peptide corresponding to amino acids 190-230 of human Wnt-5a was used as the immunogen for this antibody, GenBank no NP_003383.2.
Reactivity Notes
The amino acid sequence used as immunogen is 90% homologous in newt (Pleurodeles waltl), and 88% in Xenopus and axolotl (Ambystoma mexicanum). Reactivity to Petarus breviceps reported in verified customer review.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Wnt-5a Antibody - BSA Free
Western Blot: Wnt-5a AntibodyBSA Free [NBP2-24752]
Western Blot: Wnt-5a Antibody [NBP2-24752] - Analysis of Wnt-5a in human heart lysate in the 1) absence, 2) presence of immunizing peptide, 3) mouse and 4) rat heart![Immunohistochemistry-Frozen: Wnt-5a Antibody - BSA Free [NBP2-24752]](https://resources.bio-techne.com/images/products/Wnt-5a-Antibody-Immunohistochemistry-Frozen-NBP2-24752-img0005.jpg "Immunohistochemistry-Frozen: Wnt-5a Antibody - BSA Free [NBP2-24752]")
Immunohistochemistry-Frozen: Wnt-5a Antibody - BSA Free [NBP2-24752]
Immunohistochemistry-Frozen: Wnt-5a Antibody [NBP2-24752] - P. breviceps epithelial expression of Wnt5a at 1:100 dilution. Tissue samples Cryo-section fixed (12um slices) in OCT, washed for 10min in PBT (PBS + 0.1% tween), blocked for 1hr in 3%BSA/PBS, and then incubated with the antibody overnight at 4C. The next day washed 3x for 10min in PBT and incubated for one hr in Alexa 488 (1:500), washed 3x10min in PBS, and coverslipped. Image from verified customer review.![Immunohistochemistry-Paraffin: Wnt-5a Antibody - BSA Free [NBP2-24752]](https://resources.bio-techne.com/images/products/Wnt-5a-Antibody-Immunohistochemistry-Paraffin-NBP2-24752-img0003.jpg "Immunohistochemistry-Paraffin: Wnt-5a Antibody - BSA Free [NBP2-24752]")
Immunohistochemistry-Paraffin: Wnt-5a Antibody - BSA Free [NBP2-24752]
Immunohistochemistry-Paraffin: Wnt-5a Antibody [NBP2-24752] - Analysis of Wnt-5a in FFPE human breast tumor using an isotype control (left) and Wnt-5a antibody (right)Applications for Wnt-5a Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry-Frozen
reported by customer review
Immunohistochemistry-Paraffin
5-10 ug/ml
Western Blot
0.5-2 ug/ml
Reviewed Applications
Read 1 review rated 4 using NBP2-24752 in the following applications:
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Wnt-5a
Long Name
Wingless-type MMTV Integration Site Family, Member 5a
Alternate Names
Wnt5a
Gene Symbol
WNT5A
UniProt
Additional Wnt-5a Products
Product Documents for Wnt-5a Antibody - BSA Free
Product Specific Notices for Wnt-5a Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...