Wnt-5a Antibody (3P2E8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15684

Recombinant Monoclonal Antibody
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NBP3-15684-100ul
NBP3-15684-20ul
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3P2E8 expressed in HEK293

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Wnt-5a Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 281-380 of human Wnt-5a (P41221). RGKLVQVNSRFNSPTTQDLVYIDPSPDYCVRNESTGSLGTQGRLCNKTSEGMDGCELMCCGRGYDQFKTVQTERCHCKFHWCCYVKCKKCTEIVDQFVCK

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Wnt-5a Antibody (3P2E8)

Western Blot: Wnt-5a Antibody (3P2E8) [NBP3-15684]

Western Blot: Wnt-5a Antibody (3P2E8) [NBP3-15684]

Western Blot: Wnt-5a Antibody (3P2E8) [NBP3-15684] - Western blot analysis of extracts of various cell lines, using Wnt-5a antibody (NBP3-15684) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: Wnt-5a Antibody (3P2E8) [NBP3-15684]

Immunohistochemistry-Paraffin: Wnt-5a Antibody (3P2E8) [NBP3-15684]

Immunohistochemistry-Paraffin: Wnt-5a Antibody (3P2E8) [NBP3-15684] - Immunohistochemistry of paraffin-embedded human lung cancer using Wnt-5a Rabbit mAb (NBP3-15684) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for Wnt-5a Antibody (3P2E8)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Immunohistochemistry

1:100 - 1:1000

Immunohistochemistry-Paraffin

1:100 - 1:1000

Western Blot

1:1000 - 1:6000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Wnt-5a

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98%, 98% and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction. [provided by RefSeq]

Long Name

Wingless-type MMTV Integration Site Family, Member 5a

Alternate Names

Wnt5a

Gene Symbol

WNT5A

Additional Wnt-5a Products

Product Documents for Wnt-5a Antibody (3P2E8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Wnt-5a Antibody (3P2E8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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