VPS4B Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20881
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (96%), Mouse (96%), Rat (96%), Rhesus Macaque (99%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human VPS4B. The exact sequence is proprietary.
Reactivity Notes
Zebrafish (88%), Xenopus laevis (88%), Chicken (89%).
Localization
Prevacuolar compartment membrane; Peripheral membrane protein; Late endosome membrane; Peripheral membrane protein (Probable)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit VPS4B Antibody - BSA Free (NBP2-20881) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for VPS4B Antibody - BSA Free
![Immunohistochemistry-Paraffin: VPS4B Antibody [NBP2-20881]](https://resources.bio-techne.com/images/products/VPS4B-Antibody-Immunohistochemistry-Paraffin-NBP2-20881-img0005.jpg "Immunohistochemistry-Paraffin: VPS4B Antibody [NBP2-20881]")
Immunohistochemistry-Paraffin: VPS4B Antibody [NBP2-20881]
Immunohistochemistry-Paraffin: VPS4B Antibody [NBP2-20881] - Colon carcinoma. VPS4B antibody [N3C3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min .
Western Blot: VPS4B Antibody [NBP2-20881] -
Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with VPS4B antibody [N3C3] (NBP2-20881) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data are based on Human Protein Atlas program.
Immunocytochemistry/ Immunofluorescence: VPS4B Antibody [NBP2-20881] -
VPS4B antibody [N3C3] detects VPS4B protein at nucleus and endosome by immunofluorescent analysis.Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: VPS4B protein stained by VPS4B antibody [N3C3] (NBP2-20881) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.
Applications for VPS4B Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: VPS4B
Alternate Names
Cell migration-inducing gene 1 protein, Protein SKD1, SKD1B, SKD1cell migration-inducing 1, Suppressor of K(+) transport growth defect 1, suppressor of K+ transport defect 1, vacuolar protein sorting 4 homolog B (S. cerevisiae), vacuolar protein sorting 4B, vacuolar protein sorting 4B (yeast), vacuolar protein sorting-associated protein 4B, VPS42, VPS4-2
Gene Symbol
VPS4B
UniProt
Additional VPS4B Products
Product Documents for VPS4B Antibody - BSA Free
Product Specific Notices for VPS4B Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.