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VPS26A Antibody (CL2287) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44464

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NBP3-44464
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2287

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of O75436, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for VPS26A Antibody (CL2287) - Azide and BSA Free

VPS26A Antibody (CL2287) - Azide and BSA Free Immunohistochemistry-Paraffin: VPS26A Antibody [NBP3-44464]

Immunohistochemistry-Paraffin: VPS26A Antibody [NBP3-44464]

Analysis in human testis and skeletal muscle tissues using NBP3-44464 antibody. Corresponding VPS26A RNA-seq data are presented for the same tissues.
VPS26A Antibody (CL2287) - Azide and BSA Free Western Blot: VPS26A Antibody [NBP3-44464]

Western Blot: VPS26A Antibody [NBP3-44464]

Lane 1: Marker [kDa]
Lane 2: Human cell line U-251 MG
VPS26A Antibody (CL2287) - Azide and BSA Free Immunohistochemistry-Paraffin: VPS26A Antibody [NBP3-44464]

Immunohistochemistry-Paraffin: VPS26A Antibody [NBP3-44464]

Staining of human duodenum shows moderate to strong cytoplasmic positivity in glandular cells.

Applications for VPS26A Antibody (CL2287) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VPS26A

VPS26 belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.

Alternate Names

FLJ12930, HB58, Hbeta58, hVPS26, PEP8A, vacuolar protein sorting 26 (yeast homolog), vacuolar protein sorting 26 homolog A (S. pombe), vacuolar protein sorting 26 homolog A (yeast), vacuolar protein sorting-associated protein 26A, Vesicle protein sorting 26A, VPS26vacuolar protein sorting 26 (yeast)

Gene Symbol

VPS26A

Additional VPS26A Products

Product Documents for VPS26A Antibody (CL2287) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for VPS26A Antibody (CL2287) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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