Viral EBV IL-10 Alexa Fluor® 350-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB9151U
Conjugate
Catalog #
Key Product Details
Species Reactivity
Viral
Applications
Western Blot
Label
Alexa Fluor 350 (Excitation = 346 nm, Emission = 442 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 134206
Product Specifications
Immunogen
E. coli-derived recombinant viral EBV IL-10
Gln26-Arg170
Accession # P03180
Gln26-Arg170
Accession # P03180
Specificity
Detects viral EBV IL‑10 in direct ELISAs and Western blots. In Western blots, less than 2% cross-reactivity with IL-10 from canine, cotton rat, feline, human, mouse, porcine, or rat is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Viral EBV IL-10 Alexa Fluor® 350-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: IL-10
Interleukin 10 (IL-10) is a pleiotropic cytokine that plays an important role in regulating inflammatory and immune responses. It is a potent suppressant of proinflammatory cytokine production by monocytes/macrophages and neutrophils. It is also an inhibitor of macrophage and T cell effector functions. Homologs of mammalian IL-10 have been identified in the genome of several viruses, including Epstein-Barr virus (EBV), poxvirus Orf, baboon cytomegalovirus, and human and equine herpes virus. In the EBV genome, the BCRF1 open reading frame encodes the EBV IL-10 (vIL-10). The viral IL-10 precursor is a 170 amino acid residue (aa) protein with a putative 25 aa signal peptide that is cleaved to yield the 145 aa mature protein. The EBV IL-10 precursor shares approximately 78% and 65% amino acid sequence homology with human and mouse IL-10, respectively. Most of the deviations are localized to the signal peptide and the first 20 amino-terminal residues. Viral IL-10 is expressed during the late phase of the lytic cycle of EBV infection. Viral IL-10 is a partial agonist of mammalian IL-10 and shares many of their activities. It is likely that vIL-10 may have a role in host immune evasion.
Long Name
Interleukin 10
Alternate Names
CSIF, GVHDS, IL10, IL10A, TGIF
Entrez Gene IDs
Gene Symbol
IL10
UniProt
Additional IL-10 Products
Product Specific Notices for Viral EBV IL-10 Alexa Fluor® 350-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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