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Vinexin Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20861

Novus Biologicals, part of Bio-Techne
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NBP2-20861

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (94%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Vinexin. The exact sequence is proprietary.

Localization

Isoform Alpha: Cell junction (By similarity); Cytoplasm; cytoskeleton (By similarity); Isoform Beta: Cell junction (By similarity); Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Vinexin Antibody - BSA Free (NBP2-20861) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Vinexin Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Vinexin Antibody [NBP2-20861]

Immunocytochemistry/ Immunofluorescence: Vinexin Antibody [NBP2-20861]

Immunocytochemistry/Immunofluorescence: Vinexin Antibody [NBP2-20861] - Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SORBS3 protein stained by Vinexin antibody, Internal diluted at 1:500. Blue: Hoechst 33343 staining.
Immunohistochemistry-Paraffin: Vinexin Antibody [NBP2-20861]

Immunohistochemistry-Paraffin: Vinexin Antibody [NBP2-20861]

Immunohistochemistry-Paraffin: Vinexin Antibody [NBP2-20861] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:250 dilution.
Vinexin Antibody - BSA Free

Western Blot: Vinexin Antibody [NBP2-20861] -

Various whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Vinexin antibody [N3C2], Internal (NBP2-20861) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Applications for Vinexin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Vinexin

The SORBS3 gene encodes 2 isoforms vinexin-alpha and -beta that are vinculin (VCL; MIM 193065)-binding cytoskeletal proteins involved in focal adhesion and cell-cell adhesion (Kioka et al., 1999 [PubMed 9885244]).[supplied by OMIM]

Alternate Names

SCAM-1SH3-containing adapter molecule 1, SCAM1vinexin beta (SH3-containing adaptor molecule-1), SH3D4VINEXIN, sorbin and SH3 domain containing 3, Sorbin and SH3 domain-containing protein 3, vinexin

Gene Symbol

SORBS3

UniProt

Additional Vinexin Products

Product Documents for Vinexin Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Vinexin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.