VGLUT2 Antibody (N29/29) - Chimeric - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-52338
Recombinant Monoclonal Antibody
![Immunohistochemistry-Paraffin: VGLUT2 Antibody (N29/29) - Chimeric - BSA Free [NBP3-52338]](https://resources.bio-techne.com/images/products/nbp3-52338_-immunohistochemistry-paraffin-639168417273965730.jpg "Immunohistochemistry-Paraffin: VGLUT2 Antibody (N29/29) - Chimeric - BSA Free [NBP3-52338]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Chicken
Applications
Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Chicken IgY Clone # N29/29
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Fusion protein amino acids 501-582 (cytoplasmic C-terminus) of rat VGLUT2 (Uniprot#: Q9JI12) produced recombinantly in E. Coli
Reactivity Notes
Electric Ray
Specificity
Does not cross-react with VGlut1 or VGlut3
Clonality
Monoclonal
Host
Chicken
Isotype
IgY
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N29/29 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N29/29 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in HEK293 cells and then affinity purified from the cell culture media.
Scientific Data Images for VGLUT2 Antibody (N29/29) - Chimeric - BSA Free
Immunohistochemistry-Paraffin: VGLUT2 Antibody (N29/29) - Chimeric - BSA Free [NBP3-52338]
Formalin fixed, paraffin-embedded rat brain cerebellum region showing staining of VGLUT2 with NBP3-52338 at 1:100 dilution. DAPI nuclear stain (blue) shows cell nuclei. NBP3-52338 specifically stains the mossy fiber terminals in the granular layer and climbing climbing fiber terminals in the molecular layer of the cerebellum. Magnification 100X.![Western Blot: VGLUT2 Antibody (N29/29) - Chimeric - BSA Free [NBP3-52338]](https://resources.bio-techne.com/images/products/nbp3-52338_-western-blot-639168417273851128.png "Western Blot: VGLUT2 Antibody (N29/29) - Chimeric - BSA Free [NBP3-52338]")
Western Blot: VGLUT2 Antibody (N29/29) - Chimeric - BSA Free [NBP3-52338]
Western blotting of rat brain membrane fraction with NBP3-52338 antibody and detected with anti-chicken-HRP. This antibody recognizes endogenous VGLUT2 in the rat brain membrane fraction at ~60 kDa.![Immunohistochemistry-Paraffin: VGLUT2 Antibody (N29/29) - Chimeric - BSA Free [NBP3-52338]](https://resources.bio-techne.com/images/products/nbp3-52338_-immunohistochemistry-paraffin-639168417274076837.jpg "Immunohistochemistry-Paraffin: VGLUT2 Antibody (N29/29) - Chimeric - BSA Free [NBP3-52338]")
Immunohistochemistry-Paraffin: VGLUT2 Antibody (N29/29) - Chimeric - BSA Free [NBP3-52338]
Formalin fixed, paraffin-embedded rat brain cerebellum region showing staining of VGLUT2 with NBP3-52338 at 1:100 dilution. DAPI nuclear stain (blue) shows cell nuclei. This antibody specifically stains the mossy fiber terminals in the granular layer and climbing climbing fiber terminals in the molecular layer of the cerebellum. Magnification 400X.Applications for VGLUT2 Antibody (N29/29) - Chimeric - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:400
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
1X PBS (pH 7.4)
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: VGLUT2
Alternate Names
differentiation-associated BNPI, differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, differentiation-associated Na-dependent inorganic phosphate cotransporter, DNPI, solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6, solute carrier family 17 member 6, vesicular glutamate transporter 2, VGLUT2
Gene Symbol
SLC17A6
Additional VGLUT2 Products
Product Documents for VGLUT2 Antibody (N29/29) - Chimeric - BSA Free
Product Specific Notices for VGLUT2 Antibody (N29/29) - Chimeric - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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