VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-18644
![ELISA: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free [NBP1-18644]](https://resources.bio-techne.com/images/products/VEGFR2-KDR-Flk-1-Antibody-4H3-ELISA-NBP1-18644-img0006.jpg "ELISA: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free [NBP1-18644]")
![ELISA: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free [NBP1-18644]](https://resources.bio-techne.com/images/products/VEGFR2-KDR-Flk-1-Antibody-4H3-ELISA-NBP1-18644-img0018.jpg "ELISA: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free [NBP1-18644]")
![Immunocytochemistry/ Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free [NBP1-18644]](https://resources.bio-techne.com/images/products/VEGFR2-KDR-Flk-1-Antibody-4H3-Immunocytochemistry-Immunofluorescence-NBP1-18644-img0017.jpg "Immunocytochemistry/ Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free [NBP1-18644]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 4H3
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Recombinant human soluble extracellular KDR (D7)
Specificity
The monoclonal antibody will detect native human VEGFR-2/KDR on the surface of different human cell types.
Marker
Endothelial Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
Clone #3 changed to 4H3.
Scientific Data Images for VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free [NBP1-18644]
Immunocytochemistry/Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free [NBP1-18644] - VEGFR2/KDR/Flk-1 Antibody (4H3) [NBP1-18644] - IF double staining of human KDR in a co-culture of PAE-Flt-1, PAE-KDR and PAE-FLT-4 with (A) mouse anti-human KDR, (B) rabbit antihuman KDR (C) DAPI and (D) merged. Conjugated secondary antibody: goat anti-rabbit ALEXA Flour (1:600) and goat anti-mouse PE.![Flow Cytometry: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free [NBP1-18644]](https://resources.bio-techne.com/images/products/VEGFR2-KDR-Flk-1-Antibody-4H3-Flow-Cytometry-NBP1-18644-img0011.jpg "Flow Cytometry: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free [NBP1-18644]")
Flow Cytometry: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free [NBP1-18644]
Flow Cytometry: VEGFR2/KDR/Flk-1 Antibody (4H3) [NBP1-18644] - Analysis using the Biotin conjugate of NBP1-18644. Staining of VEGFR-2/KDR expression in HUVE cells.![Flow Cytometry: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free [NBP1-18644]](https://resources.bio-techne.com/images/products/VEGFR2-KDR-Flk-1-Antibody-4H3-Flow-Cytometry-NBP1-18644-img0008.jpg "Flow Cytometry: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free [NBP1-18644]")
Flow Cytometry: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free [NBP1-18644]
Flow Cytometry: VEGFR2/KDR/Flk-1 Antibody (4H3) [NBP1-18644] - Fig. 1: Flow analysis of VEGF R2/KDR/Flk-1 expression in primary human dermal mivrovascular endothelial cells (HDMVEC) and human umbilical vein endothelial cells (HUVEC).Applications for VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free
Application
Recommended Usage
ELISA
1-5 ug/ml
Flow Cytometry
2 - 5 ug/ml
Immunocytochemistry/ Immunofluorescence
1-5 ug/ml
Western Blot
1-2 ug/ml
Application Notes
This antibody is Cytof ready.
Formulation, Preparation, and Storage
Purification
Protein G purified
Reconstitution
Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: VEGFR2/KDR/Flk-1
Long Name
Vascular Endothelial Growth Factor Receptor 2
Alternate Names
CD309, Flk-1, Flk1, KDR, KRD1, Ly73, VEGF R2
Entrez Gene IDs
3791 (Human)
Gene Symbol
KDR
UniProt
Additional VEGFR2/KDR/Flk-1 Products
Product Documents for VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free
Product Specific Notices for VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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