VEGF-D Antibody (24GB1750)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51548

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NBP3-51548-100ul
NBP3-51548-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB1750

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all VEGF-D Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human VEGF-D (Uniprot: O43915)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VEGF-D Antibody (24GB1750)

VEGF-D Antibody (24GB1750)

Immunocytochemistry/ Immunofluorescence: VEGF-D Antibody (24GB1750)[NBP3-51548] -

Immunocytochemical staining of HepG2 cells with VEGF-D antibody (1:1,000). Nuclei were stained blue with DAPI; VEGF-D was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
VEGF-D Antibody (24GB1750)

Flow Cytometry: VEGF-D Antibody (24GB1750)[NBP3-51548] -

Validation of VEGF-D knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with VEGF-D antibody (1:2,000) and analyzed using BD flow cytometer.
VEGF-D Antibody (24GB1750)

Flow Cytometry: VEGF-D Antibody (24GB1750)[NBP3-51548] -

Flow cytometric analysis of VEGF-Dexpression in HepG2 cells using VEGF-D antibody (1:2,000). Green, isotype control; red, Vascular endothelial growth factor D.
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Applications for VEGF-D Antibody (24GB1750)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VEGF-D

Vascular Endothelial Growth Factor D (VEGFD), also known as c-fos-induced growth factor (FIGF), is a member of the VEGF family of growth factors. Vascular endothelial growth factors (VEGFs) are a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors. VEGFs stimulate endothelial cells, induce angiogenesis, promote cell migration, increase vascular permeability, and inhibit apoptosis. VEGFD is most closely related to VEGFC (23.3% amino acid sequence identity) and has a similar VEGF homology domain that spans the middle third of the precursor protein and the long N-terminal and C-terminal extensions. In adults, VEGFD is highly expressed in lung, heart, muscle, and small intestine. VEGFD expression in fibroblasts is induced by cell interaction mediated by cadherin 11. Recombinant human VEGFD is a ligand for the tyrosine kinases, VEGFR2 (Flk1) and VEGF receptor 3 (Flt4). Since VEGFR3 is strongly expressed in lymphatic endothelial cells, it is postulated that VEGFD is involved in the regulation of the growth and/or differentiation of lymphatic endothelium and thus, a mitogen for endothelial cells.

Long Name

Vascular Endothelial Growth Factor D/cFos-induced Growth Factor

Alternate Names

FIGF, VEGFD

Gene Symbol

VEGFD

Additional VEGF-D Products

Product Documents for VEGF-D Antibody (24GB1750)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for VEGF-D Antibody (24GB1750)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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