VCAM-1/CD106 Antibody (SA05-04)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67292

Recombinant Monoclonal Antibody
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NBP2-67292
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Zebrafish

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SA05-04 expressed in HEK293

Concentration

1 mg/ml

View all VCAM-1/CD106 Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human VCAM-1/CD106 aa 690-739 / 739. (SwissProt: P19320 Human; SwissProt: P29533 Mouse; SwissProt: P29534 Rat)

Localization

Membrane

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VCAM-1/CD106 Antibody (SA05-04)

Western Blot: VCAM-1/CD106 Antibody (SA05-04) [NBP2-67292]

Western Blot: VCAM-1/CD106 Antibody (SA05-04) [NBP2-67292]

Western Blot: VCAM-1/CD106 Antibody (SA05-04) [NBP2-67292] - Analysis of VCAM1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysateLane 2: human spleen tissue lysate
Immunocytochemistry/ Immunofluorescence: VCAM-1/CD106 Antibody (SA05-04) [NBP2-67292]

Immunocytochemistry/ Immunofluorescence: VCAM-1/CD106 Antibody (SA05-04) [NBP2-67292]

Immunocytochemistry/Immunofluorescence: VCAM-1/CD106 Antibody (SA05-04) [NBP2-67292] - Staining VCAM1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (SA05-04) [NBP2-67292]

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (SA05-04) [NBP2-67292]

Immunohistochemistry-Paraffin: VCAM-1/CD106 Antibody (SA05-04) [NBP2-67292] - Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-VCAM-1/CD106 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA
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Applications for VCAM-1/CD106 Antibody (SA05-04)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

1:500-1:5000
Application Notes
IP - assay-dependent

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VCAM-1/CD106

CD106 is a 110 kD glycosylphosphatidylinositol (GPI)-linked transmembrane protein, also known as VCAM-1 and INCAM-110. It is constitutively expressed on bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells, as well as some lymphocytes. CD106 expression can be upregulated on endothelial cells by inflammatory cytokines. CD106 is involved in adhesion and acts as a counter-receptor for VLA-4 ( alpha4/ beta1 integrin) and LPAM-1 ( alpha4/ beta7 integrin). The 429 antibody has been reported to partially block VCAM-1-mediated binding.

Long Name

Vascular Cell Adhesion Molecule 1

Alternate Names

CD106, VCAM1

Gene Symbol

VCAM1

Additional VCAM-1/CD106 Products

Product Documents for VCAM-1/CD106 Antibody (SA05-04)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for VCAM-1/CD106 Antibody (SA05-04)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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