VAP-1/AOC3 Antibody (174-5) - Azide Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-58375

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NBP1-58375
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 174-5

Format

Azide Free

Concentration

0.1 mg/ml

View all VAP-1/AOC3 Antibodies »

Product Specifications

Immunogen

The monoclonal antibody 174-5 cross-reacts with rat VAP-1. The monoclonal antibody inhibits lymphocyte infiltration in liver allograft rejection in rats.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for VAP-1/AOC3 Antibody (174-5) - Azide Free

Immunohistochemistry-Paraffin: VAP-1/AOC3 Antibody (174-5) [NBP1-58375]

Immunohistochemistry-Paraffin: VAP-1/AOC3 Antibody (174-5) [NBP1-58375]

Immunohistochemistry-Paraffin: VAP1 Antibody (174-5) [NBP1-58375] - Analysis of VAP1 on human tonsil tissue. Anti-human VAP1 staining results in vessels that are VAP1 positive, whereas morphologically similar vessels next to postive ones can be negative.

Applications for VAP-1/AOC3 Antibody (174-5) - Azide Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

0.2 um filtered solution in PBS and 0.1% BSA

Format

Azide Free

Preservative

No Preservative

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: VAP-1/AOC3

The monoclonal antibody 174-5 recognises human Vascular Adhesion Protein-1 (VAP-1) also known as AOC3, a heavily sialylated homodimeric glycoprotein of 180 kD. VAP-1 is present in endothelial cells, smooth muscle cells, adipocytes, and in follicular dendritic cells. The majority of VAP-1 is stored within intracellular granules and translocated to the endothelial surface on inflammation. Structurally it belongs to enzymes called semicarbamizide-sensitive amine oxidases. These enzymes deaminate primary amines in a reaction producing hydrogen peroxide, aldehyde, and ammonia. VAP-1 possesses both adhesive and enzymatic properties in vitro. VAP-1 mediates leukocyte trafficking to sites of inflammation. The monoclonal antibody 174-5 cross-reacts with rat VAP-1. The monoclonal antibody inhibits lymphocyte infiltration in liver allograft rejection in rats.

Long Name

Vascular Adhesion Protein-1

Alternate Names

AOC3, HPAO, SSAO, VAP1

Gene Symbol

AOC3

Additional VAP-1/AOC3 Products

Product Documents for VAP-1/AOC3 Antibody (174-5) - Azide Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for VAP-1/AOC3 Antibody (174-5) - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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