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USP16 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-81638

Novus Biologicals, part of Bio-Techne
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NB100-81638
Conjugate
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human ubiquitin specific peptidase 16 using the numbering given in entry NP_006438.1 (GeneID 10600).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for USP16 Antibody - BSA Free

Western Blot: USP16 Antibody [NB100-81638]

Western Blot: USP16 Antibody [NB100-81638]

Western Blot: USP16 Antibody [NB100-81638] - Detection of Human USP16 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP16 antibody NB100-81638 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: USP16 Antibody [NB100-81638]

Western Blot: USP16 Antibody [NB100-81638]

Western Blot: USP16 Antibody [NB100-81638] - Detection of Human USP16 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-USP16 antibody NB100-81638 used for IP at 3 mcg/mg lysate. USP16 was also immunoprecipitated by rabbit anti- USP16 antibody NB100-81639, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for USP16 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1:10-1:500
Application Notes
This antibody is not recommended for Western Blot analysis.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: USP16

This gene encodes a deubiquitinating enzyme that is phosphorylated at the onset of mitosis and then dephosphorylated at the metaphase/anaphase transition. It can deubiquitinate H2A, one of two major ubiquitinated proteins of chromatin, in vitro and a mutant form of the protein was shown to block cell division. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Long Name

Ubiquitin Specific Protease 16

Alternate Names

UBP-M

Entrez Gene IDs

10600 (Human)

Gene Symbol

USP16

Additional USP16 Products

Product Documents for USP16 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for USP16 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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