Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all UPF2 Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 1 and 50 of human Regulator of Nonsense Transcripts 2 using the numbering given in entry NP_056357.1 (GeneID 26019).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UPF2 Antibody - BSA Free

Western Blot: UPF2 Antibody [NBP2-22281]

Western Blot: UPF2 Antibody [NBP2-22281]

Western Blot: UPF2 Antibody [NBP2-22281] - Whole cell lysate from mouse NIH3T3 (50 ug), TCMK-1 (50 ug), and 4T1 (50 ug) cells. NBP2-22281 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Western Blot: UPF2 Antibody [NBP2-22281]

Western Blot: UPF2 Antibody [NBP2-22281]

Western Blot: UPF2 Antibody [NBP2-22281] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. NBP2-22281 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: UPF2 Antibody [NBP2-22281]

Immunoprecipitation: UPF2 Antibody [NBP2-22281]

Immunoprecipitation: UPF2 Antibody [NBP2-22281] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22281 used for IP at 6 ug/mg lysate. For blotting immunoprecipitated RENT2, NBP2-22281 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for UPF2 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 10 µg/mg lysate

Western Blot

1:1000 - 1:5000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: UPF2

UPF2 encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located in the perinuclear area. It interacts with translation release factors and the proteins that are functional homologs of yeast Upf1p and Upf3p. Two splice variants have been found for this gene; both variants encode the same protein.

Alternate Names

DKFZp434D222, hUpf2, KIAA1408MGC138835, Nonsense mRNA reducing factor 2, regulator of nonsense transcripts 2, RENT2MGC138834, smg-3, UPF2 regulator of nonsense transcripts homolog (yeast), Up-frameshift suppressor 2 homolog, yeast Upf2p homolog

Gene Symbol

UPF2

UniProt

NP_056357.1 (Human)

Additional UPF2 Products

Product Documents for UPF2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for UPF2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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