UFL1 Antibody

Western Blot: UFL1 Antibody [NBP1-79039]

Western Blot: UFL1 Antibody [NBP1-79039] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), Jurkat (J; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-ULF1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. ULF1 was also immunoprecipitated by rabbit anti-ULF1 antibody NBP1-79038 which recognizes an upstream epitope.

Western Blot: UFL1 Antibody [NBP1-79039] -

Folic acid suppresses ER-phagy, worsening ER stress, and apoptosis.A Diagrammatic representation of FA mice (250 mg/kg) and control mice. The renal function was evaluated by serum creatinine (B) and BUN (C). D Representative histology of HE and PAS staining in the renal cortex. Scale bar: 500 μm and 50 μm. E–I The immunoblot analysis and quantification of DDRGK1, UFL1, UFM1, and CANX in kidney lysates. J TEM images of ER in renal tubular epithelial cell. Red arrows: ER. K–M The immunoblot analysis and quantification of GRP78 and CHOP in kidney lysates. N, O The immunoblot analysis and quantification of BAX in kidney lysates. Data are presented as the mean +/- SEM (n = 5). **p < 0.01 and ***p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38233375), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: UFL1 Antibody [NBP1-79039] -

Confirmation of the involvement of UFL1 and UFSP2 in RPL10 ufmylation and the effect on pancreatic cancer cells.A Direct binding of RPL10 and UFL1 in PANC-1 cells was detected by Co-IP. B The change of RPL10 ufmylation was detected by after UFL1 silencing in PANC-1 cells. C Direct binding of UFSP2 and RPL10 in PANC-1 cells by Co-IP. D The change of RPL10 ufmylation after overexpressing UFSP2 in PANC-1 cells. E The proliferations of PANC-1 cells and Mia PaCa-2 cells after silencing UFL1. The proliferations were detected by CCK-8 method (n = 10). F The proliferations of PANC-1 and Mia PaCa-2 cells after overexpressing UFSP2. The proliferation rates were detected by CCK-8 method (n = 10). G The colony formation of PANC-1 and Mia PaCa-2 cells after silencing UFL1. The colonies were stained with crystal violet and analyzed (n = 3). H The colony formation of PANC-1 and Mia PaCa-2 cells after overexpressing UFSP2. The colonies were stained with crystal violet and analyzed (n = 3). All experiments were independently repeated for 3 times, and the data are expressed as mean +/- SD. *P < 0.05 indicates statistically significant versus negative control. **P < 0.01 indicates statistically very significant versus negative control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37280198), licensed under a CC-BY license. Not internally tested by Novus Biologicals.