Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunoblotting

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all UBR4 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1350 and 1400 of human retinoblastoma-associated factor 600 using the numbering given in entry NP_065816.1 (GeneID 23352).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UBR4 Antibody - BSA Free

Western Blot: UBR4 Antibody [NBP1-28730]

Western Blot: UBR4 Antibody [NBP1-28730]

Western Blot: UBR4 Antibody [NBP1-28730] - Whole cell lysate from NIH3T3, TCMK-1, 4T1, and CT26.WT cells.
Immunohistochemistry-Paraffin: UBR4 Antibody [NBP1-28730]

Immunohistochemistry-Paraffin: UBR4 Antibody [NBP1-28730]

Immunohistochemistry-Paraffin: UBR4 Antibody [NBP1-28730] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- p600 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: UBR4 Antibody [NBP1-28730]

Immunoprecipitation: UBR4 Antibody [NBP1-28730]

Immunoprecipitation: UBR4 Antibody [NBP1-28730] - Detection of Human p600 on HeLa whole cell lysate using NBP28730. p600 was also immunoprecipitated by rabbit anti-p600 antibodies NBP1-28683 and NBP1-28724.

Applications for UBR4 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 to 1:2000

Immunohistochemistry-Paraffin

1:500 to 1:2000

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: UBR4

p600 is an E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. It recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Together with clathrin, p600 forms meshwork structures involved in membrane morphogenesis and cytoskeletal organization. Additionally p600 regulates integrin-mediated signaling and may play a role in activation of FAK in response to cell-matrix interactions.

Alternate Names

600 kDa retinoblastoma protein-associated factor, EC 6.3.2, EC 6.3.2.-, KIAA0462E3 ubiquitin-protein ligase UBR4, KIAA1307zinc finger, UBR1 type 1, N-recognin-4, p600, RBAF600FLJ41863, Retinoblastoma-associated factor of 600 kDa, ubiquitin protein ligase E3 component n-recognin 4, Zinc finger UBR1-type protein 1, ZUBR1retinoblastoma-associated factor 600-like protein

Entrez Gene IDs

23352 (Human)

Gene Symbol

UBR4

UniProt

Q5T4S7 (Human)

Additional UBR4 Products

Product Documents for UBR4 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for UBR4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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