Ubiquitin-activating Enzyme/UBE1 Antibody (24GB2435)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50641
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 24GB2435
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human Ubiquitin-activating Enzyme/UBE1 (Uniprot: P22314)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Ubiquitin-activating Enzyme/UBE1 Antibody (24GB2435)
Immunocytochemistry/ Immunofluorescence: Ubiquitin-activating Enzyme/UBE1 Antibody (24GB2435)[NBP3-50641] -
Immunocytochemical staining of HepG2 cells with Ubiquitin-activating Enzyme/UBE1 antibody (1:1.000). Nuclei were stained blue with DAPI; Ubiquitin-activating Enzyme/UBE1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Flow Cytometry: Ubiquitin-activating Enzyme/UBE1 Antibody (24GB2435)[NBP3-50641] -
Flow cytometric analysis of Ubiquitin-activating Enzyme/UBE1expression in HepG2 cells using Ubiquitin-activating Enzyme/UBE1 antibody (1:2,000). Green, isotype control; red, Ubiquitin like modifier activating enzyme 1.
Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody (24GB2435)[NBP3-50641] -
Western blotting analysis using Ubiquitin-activating Enzyme/UBE1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.Applications for Ubiquitin-activating Enzyme/UBE1 Antibody (24GB2435)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Ubiquitin-activating Enzyme/UBE1
Alternate Names
A1S9T, AMCX1, GXP1, POC20, SMAX2, UBA1, UBE1, Ubiquitinactivating Enzyme
Gene Symbol
UBA1
Additional Ubiquitin-activating Enzyme/UBE1 Products
Product Documents for Ubiquitin-activating Enzyme/UBE1 Antibody (24GB2435)
Product Specific Notices for Ubiquitin-activating Enzyme/UBE1 Antibody (24GB2435)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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