UbcH5a/UBE2D1 Antibody (07) - Azide and BSA Free

Flow Cytometry: UbcH5a/UBE2D1 Antibody (07) [NBP3-06652]

Flow Cytometry: UbcH5a/UBE2D1 Antibody (07) [NBP3-06652] - Flow cytometric analysis of Human UBE2D1 expression on A431 cells. Cells were stained with purified anti-Human UBE2D1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Western Blot: UbcH5a/UBE2D1 Antibody (07) - Azide and BSA Free [NBP3-06652] -

AF enhances UBA1 interaction with most of the ubiquitin-conjugating E2s.a AF enhances UBA1 interactions with 20 E2s in cells. The heatmap was obtained by two independent ani-HA-UBA1 coIP followed by protein identification and quantification by mass spectrometry. The first four columns represent the summary p-value (columns 1,2) and fold change (columns 3,4) for control versus AF treatment groups. Columns 4–8 represent the UBA1-associated protein levels, calculated as an average from quadruplicate samples in each experimental run. b Guide tree of 36 human E2 ubiquitin-conjugating enzymes. Multiple sequence alignment and Guide tree of the 36 E2 proteins was performed by Clustal Omega39. The E2s in two clusters in red dash-line rectangles are E2s whose interactions with UBA1 were enhanced by AF as shown in (a). The three E2s underlined are exceptions. The number following the E2s is indicative of the evolutionary distance between the sequences. c Validation of AF-enhanced UBA1-E2 interactions in cells by anti-HA-UBA1 coIP as in (a) followed by IB. d Validation of AF-enhanced UBA1-E2 interactions in cells by anti-UBA1 coIP as in (a) followed by IB. e C1039A mutation diminishes UBA1 interactions with E2s. f AF does not promote the UBA6 interaction with UBE2Z in a GST-UBA6 pull-down assay. g AF does not affect the UBA2 interaction with UBE2I as revealed by anti-HA-UBE2I coIP. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37558718), licensed under a CC-BY license. Not internally tested by Novus Biologicals.