Tyrosine Hydroxylase Antibody (OTI2E6)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44652
![Tyrosine Hydroxylase Antibody (OTI2E6) Immunocytochemistry/ Immunofluorescence;Tyrosine Hydroxylase Antibody (OTI2E6)[NBP3-44652]-](https://resources.bio-techne.com/images/products/nbp3-44652_mouse-tyrosine-hydroxylase-mab-oti2e6-azide-and-bsa-free-immunocytochemistry-immunofluorescence-7520262375910.jpg "Immunocytochemistry/ Immunofluorescence;Tyrosine Hydroxylase Antibody (OTI2E6)[NBP3-44652]-")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # OTI2E6
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Full length human recombinant protein of human TH(NP_000351) produced in HEK293T cell.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Tyrosine Hydroxylase Antibody (OTI2E6)
Immunocytochemistry/ Immunofluorescence;Tyrosine Hydroxylase Antibody (OTI2E6)[NBP3-44652]-
Anti-TH mouse monoclonal antibody immunofluoreaining of COS7 cells transiently transfected by pCMV6-ENTRY TH .![Tyrosine Hydroxylase Antibody (OTI2E6) Western Blot;Tyrosine Hydroxylase Antibody (OTI2E6)[NBP3-44652]-](https://resources.bio-techne.com/images/products/nbp3-44652_mouse-tyrosine-hydroxylase-mab-oti2e6-azide-and-bsa-free-western-blot-752026224544.jpg "Western Blot;Tyrosine Hydroxylase Antibody (OTI2E6)[NBP3-44652]-")
Western Blot;Tyrosine Hydroxylase Antibody (OTI2E6)[NBP3-44652]-
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TH (, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TH.Applications for Tyrosine Hydroxylase Antibody (OTI2E6)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:4000
Formulation, Preparation, and Storage
Purification
>95%
Reconstitution
For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized powder ( 1X PBS, pH 7.3,)
Preservative
8% Trehalose
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 degrees C. Avoid freeze/thaw cycles.
Background: Tyrosine Hydroxylase
Two transcription factor binding sites in the proximal region of the TH gene, the TPA-responsive element (TRE) and the c-AMP responsive element (CRE), have been implicated in the complex regulation of the TH gene. Dysregulation of breakdown for the amino acid, tyrosine, by TH is a result of a genetic disorder that results in Tyrosinemia (high levels of tyrosine in the blood, tissue and organs).
Tyrosine hydroxylase deficiency is a disorder that primarily affects movement, where individuals display symptoms that include lack of coordination when walking, postural tremors and unusual body positioning. TH deficient dopamine-responsive dystonia (DRD), also known as Segawa syndrome, is a rare genetic disorder that is associated with low levels of TH and is diagnosed during childhood with characteristic symptoms including increased muscle tone (dystonia) and signs of Parkinsonism like bradykinesia, tremors, rigidity and postural instability (2). Correspondingly, TH is also linked to Parkinson's disease in older adults, where low dopamine levels are a consistent neurochemical abnormality. Functional polymorphisms of the TH gene may be involved in the pathogenesis of neuropsychiatric diseases such as schizophrenia and other affective disorders where dopamine is often dysregulated (3).
References
1. Hamanaka, Y., & Mizunami, M. (2019). Tyrosine hydroxylase-immunoreactive neurons in the mushroom body of the field cricket, Gryllus bimaculatus. Cell Tissue Res, 376(1), 97-111. doi:10.1007/s00441-018-2969-9
2. Li, L., & Zhou, F. M. (2013). Parallel dopamine D1 receptor activity dependence of l-Dopa-induced normal movement and dyskinesia in mice. Neuroscience, 236, 66-76. doi:10.1016/j.neuroscience.2012.12.065
3. Borkar, C. D., Bharne, A. P., Nagalakshmi, B., Sakharkar, A. J., Subhedar, N. K., & Kokare, D. M. (2018). Cocaine- and Amphetamine-Regulated Transcript Peptide (CART) Alleviates MK-801-Induced Schizophrenic Dementia-Like Symptoms. Neuroscience, 375, 94-107. doi:10.1016/j.neuroscience.2018.01.056
Alternate Names
TH, TYH
Gene Symbol
TH
Additional Tyrosine Hydroxylase Products
Product Documents for Tyrosine Hydroxylase Antibody (OTI2E6)
Product Specific Notices for Tyrosine Hydroxylase Antibody (OTI2E6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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