Tryptophan hydroxylase 2 Antibody (CL2990) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-57829

Recombinant Monoclonal Antibody
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NBP3-57829-25ul
NBP3-57829-100ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # CL2990

Format

BSA Free

Concentration

1 mg/ml

View all Tryptophan Hydroxylase 2 Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q8IWU9, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Tryptophan hydroxylase 2 Antibody (CL2990) - BSA Free

Tryptophan hydroxylase 2 Antibody (CL2990) - BSA Free

Tryptophan hydroxylase 2 Antibody (CL2990) - BSA Free

Staining of human brain shows strong cytoplasmic positivity in the dorsal raphe neurons.
Tryptophan hydroxylase 2 Antibody (CL2990) - BSA Free

Tryptophan hydroxylase 2 Antibody (CL2990) - BSA Free

Staining of human cerebral cortex shows moderate positivity in serotonergic neural fibers.
Tryptophan hydroxylase 2 Antibody (CL2990) - BSA Free

Tryptophan hydroxylase 2 Antibody (CL2990) - BSA Free

Staining of human dorsal raphe nucleus shows strong cytoplasmic positivity in serotonin neurons.
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Applications for Tryptophan hydroxylase 2 Antibody (CL2990) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2), 40% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Tryptophan Hydroxylase 2

Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996). CaM K II phosphorylates Ser260 which lies within the regulatory domain of TPH (Jiang et al., 2000).

Alternate Names

NTPH, TPH2

Gene Symbol

TPH2

Additional Tryptophan Hydroxylase 2 Products

Product Documents for Tryptophan hydroxylase 2 Antibody (CL2990) - BSA Free

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Product Specific Notices for Tryptophan hydroxylase 2 Antibody (CL2990) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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