TRPM8 Antibody - BSA Free

Immunohistochemistry: TRPM8 Antibody - BSA Free [NBP1-97311]

Immunohistochemistry: TRPM8 Antibody [NBP1-97311] - Analysis of TRPM8 in mouse prostate using DAB with hematoxylin counterstain.

Western Blot: TRPM8 AntibodyBSA Free [NBP1-97311]

Western Blot: TRPM8 Antibody [NBP1-97311] - Analysis of TRPM8 in PC12 cell lysate.

Immunocytochemistry/ Immunofluorescence: TRPM8 Antibody - BSA Free [NBP1-97311]

Immunocytochemistry/Immunofluorescence: TRPM8 Antibody [NBP1-97311] - TRPM8 antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Immunocytochemistry/ Immunofluorescence: TRPM8 Antibody - BSA Free [NBP1-97311] -

Immunocytochemistry/ Immunofluorescence: TRPM8 Antibody - BSA Free [NBP1-97311] - Confocal imaging of TRPM8, NF kappaB & TNF alpha expression in PC12 cells.(A) The expression of TRPM8, NF kappaB & TNF alpha in wild type cells & Trpm8 knockdown cells at 37 °C & 4 °C. KD signifies Trpm8 knockdown cells. (B) In wild type cells, TRPM8 was upregulated & NF kappaB & TNF alpha were downregulated under cold conditions. (C) In KD cells, TRPM8 showed weak expression & NF kappaB & TNF alpha expression levels were increased. (D) Co-localization of TRPM8 & TNF alpha in the cytoplasm (cold condition & 500 nM menthol). Data are shown as the mean ± S.D. from three experiments. *P < 0.05; **P < 0.01. Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/srep45155), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: TRPM8 Antibody - BSA Free [NBP1-97311] -

Western Blot: TRPM8 Antibody - BSA Free [NBP1-97311] - Expression of TRPM8, TRPA1, NF kappaB & TNF alpha in PC12 cells with Trpm8 knockdown under cold conditions (4 °C).(A) Construction of a Trpm8 (Dylight 649) knockdown stable cell line. WT represents wild type cells. KD signifies the Trpm8 knockdown cells. (B,C) Protein expression levels of TRPM8, TRPA1, NF kappaB & TNF alpha. NS: no significance. Data are shown as the mean ± S.D. from three experiments. #P < 0.05; ##P < 0.01, v.s. the control (zero time). Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/srep45155), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: TRPM8 Antibody - BSA Free [NBP1-97311] -

Western Blot: TRPM8 Antibody - BSA Free [NBP1-97311] - Alteration of core temperature & the expression levels of TRPM8, TRPA1, NF kappaB & TNF alpha in mouse brains under cold conditions.(A) Core body temperature under cold conditions (4 °C). (B) The mRNA expression levels of TRPM8, TRPA1, NF kappaB & TNF alpha. (C,D) The protein expression levels of TRPM8, TRPA1, NF kappaB & TNF alpha. Data are shown as the mean ± S.D. from 12 mice in each group. ##v.s. the control (zero hour), P < 0.01; #P < 0.05. Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/srep45155), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: TRPM8 Antibody - BSA Free [NBP1-97311] -

Immunocytochemistry/ Immunofluorescence: TRPM8 Antibody - BSA Free [NBP1-97311] - Co-localization of TRPM8 & NF kappaB in PC12 cells under cold conditions.(A) Immunofluorescence assay image of TRPM8 & NF kappaB in the cytoplasm. WT represents wild type cells. KD represents Trpm8 knockdown cells. (B) The results of co-immunoprecipitation (CoIP) of endogenous TRPM8 & NF kappaB using NF kappaB antibodies. Western blot analysis was carried out to detect TRPM8 & NF kappaB. (C) Reverse CoIP confirmed interaction between NF kappaB & TRPM8. CoIPs were also performed with TRPM8 antibodies. Western blot analysis was carried out by TRPM8 & NF kappaB. Data are shown as the mean ± S.D. from three experiments. *P < 0.05; **P < 0.01. Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/srep45155), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: TRPM8 Antibody - BSA Free [NBP1-97311] -

Western Blot: TRPM8 Antibody - BSA Free [NBP1-97311] - Expression of TRPM8, TRPA1, NF kappaB & TNF alpha in PC12 cells under cold conditions.(A) Intracellular Ca2+ in the cells under cold conditions (4 °C). The Ca2+ concentration in the cytoplasm at 4 °C is higher than that at 37 °C. (B) The mRNA expression levels of TRPM8, TRPA1, NF kappaB & TNF alpha. (C,D) The protein expression levels of TRPM8, TRPA1, NF kappaB & TNF alpha. Data are shown as the mean ± S.D. from three experiments. #P < 0.05; ##P < 0.01, v.s. the control (zero time). Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/srep45155), licensed under a CC-BY license. Not internally tested by Novus Biologicals.