TRMT5 Antibody (OTI1B5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44733

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NBP3-44733
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1B5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TRMT5 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-303 of human TRMT5(NP_065861) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for TRMT5 Antibody (OTI1B5)

TRMT5 Antibody (OTI1B5) Western Blot;TRMT5 Antibody (OTI1B5)[NBP3-44733]-

Western Blot;TRMT5 Antibody (OTI1B5)[NBP3-44733]-

HEK293T cells were transfected with the pCMV6-ENTRY control ( Left lane) or pCMV6-ENTRY TRMT5 ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRMT5 (1:2000). Positive lysates
TRMT5 Antibody (OTI1B5) Immunohistochemistry;TRMT5 Antibody (OTI1B5)[NBP3-44733]-

Immunohistochemistry;TRMT5 Antibody (OTI1B5)[NBP3-44733]-

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TRMT5 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
TRMT5 Antibody (OTI1B5) Immunohistochemistry;TRMT5 Antibody (OTI1B5)[NBP3-44733]-

Immunohistochemistry;TRMT5 Antibody (OTI1B5)[NBP3-44733]-

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TRMT5 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
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Applications for TRMT5 Antibody (OTI1B5)

Application
Recommended Usage

Immunohistochemistry

1:1000

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

>95%

Reconstitution

For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized powder ( 1X PBS, pH 7.3,)

Preservative

8% Trehalose

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 degrees C. Avoid freeze/thaw cycles.

Background: TRMT5

tRNAs contain as many as 13 or 14 nucleotides that are modified posttranscriptionally by enzymes that are highly specific for particular nucleotides in the tRNA structure. TRMT5 methylates the N1 position of guanosine-37 (G37) in selected tRNAs using S-adenosyl methionine (Brule et al., 2004 (PubMed 15248782)).(supplied by OMIM)

Alternate Names

KIAA1393EC 2.1.1.31, M1G-methyltransferase, TRM5 tRNA methyltransferase 5 homolog (S. cerevisiae), TRM5MGC111453, tRNA (guanine-N(1)-)-methyltransferase, tRNA [GM37] methyltransferase, tRNA methyltransferase 5, tRNA-N1G37 methyltransferase

Gene Symbol

TRMT5

Additional TRMT5 Products

Product Documents for TRMT5 Antibody (OTI1B5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TRMT5 Antibody (OTI1B5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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