Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all TRERF1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 350 and 400 of human transcriptional regulating protein 132 (transcriptional regulating factor 1) using the numbering given in entry NP_277037.1 (GeneID 55809).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRERF1 Antibody - BSA Free

Immunoprecipitation: TRERF1 Antibody [NB100-81654]

Immunoprecipitation: TRERF1 Antibody [NB100-81654]

Immunoprecipitation: TRERF1 Antibody [NB100-81654] - Detection of human TRERF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRERF1 antibody NB100-81654 used for IP at 3 ug per reaction. TRERF1 was also immunoprecipitated by rabbit anti-TRERF1 antibody NB100-81655. For blotting immunoprecipitated TRERF1, NB100-81655 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for TRERF1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-5 ug/mg lysate
Application Notes
This antibody can be used for Co-IP application (PMID: 21258344). It is not recommended for Western Blot analysis.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TRERF1

The transcriptional regulating protein of 132 kDa (TReP-132/TRERF1) was identified as a transcription factor that interacts with and activates the promoter of the CYP11A1 gene, a gene that encodes cytochrome P450 side chain cleavage (P450scc) and catalyzes the first step in steroidgenesis. TRep-132 can function as a nuclear receptor co-activator and has been shown to interact with Sp1 and the progesterone receptor (PR) to suppress growth via activation of the gene promoters of the cyclin-dependent kinase inhibitors, p21 and p27.

Alternate Names

BCAR2, Breast cancer estrogen resistance 2RAPAHSA277276, dJ139D8.5, FLJ21198, rapa-1, rapa-2, RP1-139D8.5, transcriptional regulating factor 1, transcriptional regulating protein 132, transcriptional-regulating factor 1, Transcriptional-regulating protein 132, TREP132, TReP-132, Zinc finger protein rapa, Zinc finger transcription factor TReP-132

Entrez Gene IDs

55809 (Human)

Gene Symbol

TRERF1

UniProt

Q96PN7 (Human)

Additional TRERF1 Products

Product Documents for TRERF1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TRERF1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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