TRBC1 Antibody (38C2) - Chimeric - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43577
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 38C2
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
The original antibody was generated by immunizing mice with TCRbetaF1 protein.
Specificity
The antibody is specific for TCRbetaF1.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.
Scientific Data Images for TRBC1 Antibody (38C2) - Chimeric - BSA Free
Western Blot: TRBC1 Antibody (38C2) - Chimeric - BSA Free [NBP3-43577] -
Western Blot: TRBC1 Antibody (38C2) - Chimeric - BSA Free [NBP3-43577] - Jurkat (A) and Molt4 (B) cells lysates (35ug protein in RIPA buffer) were resolved via SDS-PAGE, and the subsequent blots were probed with NBP3-43577 at 0.1ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.
Immunocytochemistry /Immunofluorescence: TRBC1 Antibody (38C2) - Chimeric - BSA Free [NBP3-43577] -
Immunocytochemistry /Immunofluorescence: TRBC1 Antibody (38C2) - Chimeric - BSA Free [NBP3-43577] - Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix coverslips stained with NBP3-43577 (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-43577, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.Applications for TRBC1 Antibody (38C2) - Chimeric - BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TRBC1
Alternate Names
BV05S1J2.2, TCRBC1, TRBC1 T cell receptor beta constant 1
Gene Symbol
TRBC1
Additional TRBC1 Products
Product Documents for TRBC1 Antibody (38C2) - Chimeric - BSA Free
Product Specific Notices for TRBC1 Antibody (38C2) - Chimeric - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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