TRACP/PAP/ACP5 Antibody (ACP5/1070) [DyLight 680]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47666FR
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
DyLight 680 (Excitation = 692 nm, Emission = 712 nm)
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # ACP5/1070
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant full-length human TRACP/PAP/ACP5 protein (Uniprot: P13686)
Localization
Cytoplasmic (Lysosomes)
Specificity
It recognizes a protein of 35kDa, which is identified as tartrate-resistant acid phosphatase (TRAcP). It exists as two isoforms (5a and 5b). This monoclonal antibody reacts with both the isoforms. Serum TRAcP 5a is secreted by macrophages and dendritic cells and increased in many patients of rheumatoid arthritis.Serum TRAcP 5b is produced from osteoclasts and elevated during bone resorption. TRAcP is an iron containing glycoprotein, which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. TRAcP is synthesized as a latent proenzyme and is activated by proteolytic cleavage and reduction. Normally, TRAcP is highly expressed by osteoclasts, activated macrophages, neurons and endometrium during pregnancy. Expression of TRAcP is increased in certain pathological conditions such as Leukemic Reticuloendotheliosis (Hairy Cell Leukemia), Gauchers Disease, HIV-induced Encephalopathy, Osteoclastoma and in osteoporosis and metabolic bone diseases. Anti-TRAcP antibody labels the cells of Hairy Cell Leukemia (HCL) with a high degree of sensitivity and specificity. Other cells stained with this antibody are tissue macrophages and osteoclasts.
Marker
Hairy Cell Leukemia Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Applications for TRACP/PAP/ACP5 Antibody (ACP5/1070) [DyLight 680]
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: TRACP/PAP/ACP5
TRACP is an iron-containing, 35 kDa glycoprotein that hydrolyses phosphomonoesters. It is produced as a monomer by osteoclasts and is cleaved by cathepsins to create a disulfide-linked dimer that acts on bone matrix phosphoserine-containing proteins in lysosomal vesicles. Following phosphate cleavage, TRACP is released from the cell where it serves as a marker of bone resorption.
Long Name
Tartrate Resistant Acid Phosphatase 5
Alternate Names
ACP5, TRACP-5b, TTRRAP
Gene Symbol
ACP5
Additional TRACP/PAP/ACP5 Products
Product Documents for TRACP/PAP/ACP5 Antibody (ACP5/1070) [DyLight 680]
Product Specific Notices for TRACP/PAP/ACP5 Antibody (ACP5/1070) [DyLight 680]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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