TRA-1-85/CD147 Antibody (TRA-1-85) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # FAB3195CL1
![TRA-1-85/CD147 Antibody (TRA-1-85) [CoraFluor™ 1]](https://resources.bio-techne.com/images/products/fab3195cl1_mouse-tra-1-85-cd147-mab-tra-1-85-corafluor-1-259202511191421.png "Product Feature: CoraFluor Probes for TR-FRET")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Knockout Validated, Western Blot, Flow Cytometry, Immunocytochemistry, CyTOF-ready
Label
CoraFluor 1
Antibody Source
Monoclonal Mouse IgG1 Clone # TRA-1-85
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
2120Ep human embryonal carcinoma cell line
Specificity
Detects human TRA‑1‑85 antigen in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
Scientific Data Images for TRA-1-85/CD147 Antibody (TRA-1-85) [CoraFluor™ 1]
Product Feature: CoraFluor Probes for TR-FRET
CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.Applications for TRA-1-85/CD147 Antibody (TRA-1-85) [CoraFluor™ 1]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry
Optimal dilutions of this antibody should be experimentally determined.
Knockout Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: TRA-1-85/CD147
The TRA-1-85 antigen, also known as OKa blood group antigen, is a specific epitope within the protein known as basigin, EMMPRIN, or CD147. It is a cell surface determinant expressed on almost all human cell types. Antibodies to TRA-1-85 have been used in somatic cell hybrid or transplantation studies to identify tissues containing cells of human origin.
Alternate Names
OKa Blood Group Antigen, TRA185
Gene Symbol
BSG
Additional TRA-1-85/CD147 Products
Product Documents for TRA-1-85/CD147 Antibody (TRA-1-85) [CoraFluor™ 1]
Product Specific Notices for TRA-1-85/CD147 Antibody (TRA-1-85) [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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