TOR/mTOR Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-240
![Western Blot: TOR/mTOR Antibody [NB100-240]](https://resources.bio-techne.com/images/products/TOR-mTOR-Antibody-Western-Blot-NB100-240-img0016.jpg "Western Blot: TOR/mTOR Antibody [NB100-240]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human, Rat
Cited:
Human, Mouse, Rat
Predicted:
Mouse (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Cited:
Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 200 and 250 of human Mammalian Target of Rapamycin using the numbering given in entry NP_004949.1 (GeneID 2475).
Reactivity Notes
Rat reactivity reported in scientific literature (PMID: 29728793).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit TOR/mTOR Antibody - BSA Free (NB100-240) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-TOR/mTOR Antibody: Cited in 11 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for TOR/mTOR Antibody - BSA Free
Western Blot: TOR/mTOR Antibody [NB100-240]
Western Blot: TOR/mTOR Antibody [NB100-240] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, Hep-G2, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-mTOR antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunoprecipitation: TOR/mTOR Antibody [NB100-240]](https://resources.bio-techne.com/images/products/TOR-mTOR-Antibody-Immunoprecipitation-NB100-240-img0015.jpg "Immunoprecipitation: TOR/mTOR Antibody [NB100-240]")
Immunoprecipitation: TOR/mTOR Antibody [NB100-240]
Immunoprecipitation: TOR/mTOR Antibody [NB100-240] - Detection of human mTOR by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-mTOR antibody NB100-240 used for IP at 3 ug per reaction. mTOR was also immunoprecipitated by rabbit anti-mTOR antibody NB100-241. For blotting immunoprecipitated mTOR, NB100-240 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for TOR/mTOR Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:10-1:500
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:100-1:2000
Application Notes
Use in Immunohistochemistry-paraffin reported in scientific literature (Suzuki H et al).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: TOR
Long Name
Mammalian Target of Rapamycin
Alternate Names
FRAP1, FRAP2, MTOR, RAFT1
Gene Symbol
MTOR
Additional TOR Products
Product Documents for TOR/mTOR Antibody - BSA Free
Product Specific Notices for TOR/mTOR Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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