TOR/mTOR [p Ser2448] Antibody (24GB330)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51433

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NBP3-51433-100ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB330

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

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Product Specifications

Immunogen

A synthesized peptide derived from human Phospho-TOR/mTOR (S2448) (Uniprot: P42345)

Modification

p Ser2448

Specificity

This antibody detects endogenous levels of MTOR only when phosphorylated at S2448

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TOR/mTOR [p Ser2448] Antibody (24GB330)

TOR/mTOR [p Ser2448] Antibody (24GB330)

Immunocytochemistry/ Immunofluorescence: TOR/mTOR [p Ser2448] Antibody (24GB330)[NBP3-51433] -

Immunocytochemical staining of HeLa cells with TOR/mTOR antibody (1:1,000). Nuclei were stained blue with DAPI; Phospho-mTOR (S2448) was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
TOR/mTOR [p Ser2448] Antibody (24GB330)

Flow Cytometry: TOR/mTOR [p Ser2448] Antibody (24GB330)[NBP3-51433] -

Validation of TOR/mTOR knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Phospho-TOR/mTOR (S2448) antibody (1:2,000) and analyzed using BD flow cytometer.
TOR/mTOR [p Ser2448] Antibody (24GB330)

Flow Cytometry: TOR/mTOR [p Ser2448] Antibody (24GB330)[NBP3-51433] -

Flow cytometric analysis of TOR/mTORexpression in HeLa cells using TOR/mTOR antibody (1:2,000). Green, isotype control; red, Phospho-mTOR (S2448).
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Applications for TOR/mTOR [p Ser2448] Antibody (24GB330)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TOR

The mammalian target of rapamycin (mTOR) is a serine/threonine protein kinase which helps to mediate many cellular processes. Specifically, mTOR is invloved in the cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex, making mTOR antibodies useful tools for a number of important research areas.

Long Name

Mammalian Target of Rapamycin

Alternate Names

FRAP1, FRAP2, MTOR, RAFT1

Gene Symbol

MTOR

Additional TOR Products

Product Documents for TOR/mTOR [p Ser2448] Antibody (24GB330)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for TOR/mTOR [p Ser2448] Antibody (24GB330)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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