TLR1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56563

Novus Biologicals, part of Bio-Techne
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NB100-56563
NB100-56563SS
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Dot Blot, Flow (Cell Surface), Flow (Intracellular), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Cytometric Bead Assay Standard, Flow Cytometry, Flow Cytometry Control, IF/IHC, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all TLR1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a KLH-conjugated synthetic peptide corresponding to a sequence within amino acids 400-450 of human TLR1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TLR1 Antibody - BSA Free

Flow Cytometry: TLR1 Antibody [NB100-56563]

Flow Cytometry: TLR1 Antibody [NB100-56563]

TLR1-Antibody-Flow-Cytometry-NB100-56563-img0016.jpg
Western Blot: TLR1 Antibody [NB100-56563]

Western Blot: TLR1 Antibody [NB100-56563]

Western Blot: TLR1 Antibody [NB100-56563] - analysis of TLR1 in Ramos (A), Raw (B), and (C) TLR1 transfected cell lysate using this antibody. goat anti-rabbit HRP secondary antibody was used.
Western Blot: TLR1 Antibody [NB100-56563]

Western Blot: TLR1 Antibody [NB100-56563]

TLR1-Antibody-Western-Blot-NB100-56563-img0014.jpg
View 4 more images

Applications for TLR1 Antibody - BSA Free

Application
Recommended Usage

Flow (Cell Surface)

reported in scientific literature (Wong et al (2007); PMID 23593278)

Flow (Intracellular)

0.5-1 ug/1 million cells

Flow Cytometry

2-4 ug/ 1x10-6 cells

Immunohistochemistry

reported in scientific literature (PMID 27008696)

Immunohistochemistry-Paraffin

10 ug/ml

Western Blot

1-3 ug/ml
Application Notes
In Ramos, an 87 kDa band is observed. Flow (Intracellular) reported in scientific literature (PMID: 23593278)

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TLR1

The Toll-like receptor (TLR) family in mammals are comprised of a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and an IL-1 receptor motif in the cytoplasmic domain. The TLRs could constitute an important and unrecognized component of innate immunity in humans. In mice, TLR1 interacts with TLR2 and coexpression of TLR1 and TLR2 enhance the NF-kappaB activation in response to a synthetic lipopeptide. They recognize the lipid configuration of the native mycobacterial lipoprotein as well as several triacylated lipopeptides.

Long Name

Toll-like Receptor 1

Alternate Names

CD281

Entrez Gene IDs

7096 (Human); 305354 (Rat)

Gene Symbol

TLR1

UniProt

NP_003254

Additional TLR1 Products

Product Documents for TLR1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TLR1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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